EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.43 | Ca2+ | 49% inhibition at 1 mM | Methylococcus capsulatus | |
4.1.2.43 | Cu2+ | 24% inhibition at 1 mM | Methylococcus capsulatus | |
4.1.2.43 | additional information | not inhibited by ATP, ADP, AMP, NADH, D-fructose 6-phosphate, and phosphoenolpyruvate | Methylococcus capsulatus | |
4.1.2.43 | Ni2+ | 64% inhibition at 1 mM | Methylococcus capsulatus | |
5.3.1.27 | Co2+ | 1 mM, 35% residual activity | Methylococcus capsulatus | |
5.3.1.27 | Cu2+ | 1 mM, 8% residual activity | Methylococcus capsulatus | |
5.3.1.27 | Mg2+ | 5 mM, 30% loss of activity | Methylococcus capsulatus | |
5.3.1.27 | additional information | at 1 mm sugar phosphate concentration, D-allulose 6-phosphate, D-fructose 6-phosphate, 6-phospho-D-gluconate, D-ribulose 5-phosphate, D-xylulose 5-phosphate, D-erythrose 4-phosphate and glyceraldehyde 3-phosphate do not affect the isomerase activity | Methylococcus capsulatus | |
5.3.1.27 | Ni2+ | 1 mM, 14% residual activity | Methylococcus capsulatus | |
5.3.1.27 | Zn2+ | 1 mM, 40% residual activity | Methylococcus capsulatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.43 | 0.075 | - |
D-arabino-hex-3-ulose 6-phosphate | in 50 mM sodium potassium phosphate buffer, 5 mM MgCl2, at pH 7.0, at 30°C | Methylococcus capsulatus | |
4.1.2.43 | 0.083 | - |
D-ribulose 5-phosphate | in 50 mM sodium potassium phosphate buffer, 5 mM MgCl2, at pH 7.0, at 37°C | Methylococcus capsulatus | |
4.1.2.43 | 0.49 | - |
formaldehyde | in 50 mM sodium potassium phosphate buffer, 5 mM MgCl2, at pH 7.0, at 37°C | Methylococcus capsulatus | |
5.3.1.27 | 0.1 | - |
D-arabino-3-hexulose 6-phosphate | 30°C, pH 7.0 | Methylococcus capsulatus | |
5.3.1.27 | 1.1 | - |
D-fructose 6-phosphate | 30°C, pH 7.0 | Methylococcus capsulatus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.43 | Co2+ | 17% as effective as Mg2+ or Mn2+ in promoting synthase activity | Methylococcus capsulatus | |
4.1.2.43 | Mg2+ | 5 mM, essential for activity and stability, 3-hexulose phosphate synthase is rapidly and apparently irreversibly denatured by storage in the absence of one of Mg2+ | Methylococcus capsulatus | |
4.1.2.43 | Mn2+ | essential for activity and stability | Methylococcus capsulatus | |
4.1.2.43 | Zn2+ | 10% as effective as Mg2+ or Mn2+ in promoting synthase activity | Methylococcus capsulatus | |
5.3.1.27 | additional information | no requirement for divalent cations | Methylococcus capsulatus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.43 | 49000 | - |
6 * 49000, gel filtration, at pH 4.6 | Methylococcus capsulatus |
4.1.2.43 | 310000 | - |
gel filtration, under conditions of low pH or low ionic strength | Methylococcus capsulatus |
5.3.1.27 | 67000 | - |
gel filtration | Methylococcus capsulatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.43 | Methylococcus capsulatus | - |
- |
- |
5.3.1.27 | Methylococcus capsulatus | - |
grown on methane | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.43 | ammonium sulfate precipitation, DEAE-cellulose column chromatography | Methylococcus capsulatus |
5.3.1.27 | - |
Methylococcus capsulatus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.2.43 | 1.71 | - |
cell free extract, at 30°C | Methylococcus capsulatus |
4.1.2.43 | 69 | - |
after 41fold purification, at 30°C | Methylococcus capsulatus |
5.3.1.27 | 1560 | - |
30°C, pH 7.0 | Methylococcus capsulatus |
EC Number | Storage Stability | Organism |
---|---|---|
4.1.2.43 | -15°C, in the presence of 2.5 mM MgCl2, 6 months, remains stable unless repeatedly frozen and thawed | Methylococcus capsulatus |
5.3.1.27 | -15°C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months | Methylococcus capsulatus |
5.3.1.27 | 0-4°C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months | Methylococcus capsulatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.43 | D-ribulose 5-phosphate + formaldehyde | - |
Methylococcus capsulatus | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
5.3.1.27 | D-arabino-3-hexulose 6-phosphate | - |
Methylococcus capsulatus | D-fructose 6-phosphate | - |
r | |
5.3.1.27 | D-fructose 6-phosphate | - |
Methylococcus capsulatus | D-arabino-3-hexulose 6-phosphate | - |
r | |
5.3.1.27 | additional information | enzyme is specific for substrates D-arabino-3-hexulose 6-phosphate and D-fructose 6-phosphate | Methylococcus capsulatus | ? | - |
? | |
5.3.1.27 | additional information | no substrate: D-ribulose 5-phosphate, D-xylulose 5-phosphate or D-allulose 6-phosphate | Methylococcus capsulatus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.43 | homohexamer | 6 * 49000, gel filtration, at pH 4.6 | Methylococcus capsulatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.43 | 3-hexulose phosphate synthase | - |
Methylococcus capsulatus |
4.1.2.43 | D-arabino-3-hexulose 6-phosphate formaldehyde lyase | - |
Methylococcus capsulatus |
4.1.2.43 | HPS | - |
Methylococcus capsulatus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.43 | 60 | - |
3-hexulose phosphate synthase is rapidly inactivated at elevated temperatures, activity is totally lost within 5 min at 60°C | Methylococcus capsulatus |
5.3.1.27 | 60 | - |
10 min, complete loss of activity | Methylococcus capsulatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.43 | 7 | - |
- |
Methylococcus capsulatus |
5.3.1.27 | 8.3 | - |
- |
Methylococcus capsulatus |