Literature summary extracted from
Varga, B.; Barabas, O.; Takacs, E.; Nagy, N.; Nagy, P.; Vertessy, B.G.
Active site of mycobacterial dUTPase: structural characteristics and a built-in sensor (2008), Biochem. Biophys. Res. Commun., 373, 8-13.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.6.1.23 |
drug development |
dUTPase is a promising antituberculotic drug target |
Mycobacterium tuberculosis |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.6.1.23 |
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
Mycobacterium tuberculosis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.6.1.23 |
dUTPase in complex with the isosteric substrate analogue, alpha,beta-imido-dUTP, and Mg2+, about 0.223 mM dUTPase, 1.25 mM alpha,beta-imido-dUTP and 10 mM MgCl2 in 10 mM Tris-HCl, pH7.0, 50 mM NaCl, and 0.1 mM TCEP buffer is mixed with different reservoir solutions, X-ray diffraction structure determination and analysis at 1.5 A resolution, molecular replacement |
Mycobacterium tuberculosis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.6.1.23 |
H145W |
site-directed mutagenesis, the replacement introduces a 244 sensitive fluorescent label into the binding site. The steady-state kinetics show no difference in the case of the mutant and wild-type enzyme |
Mycobacterium tuberculosis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.6.1.23 |
additional information |
fast screening for binding of potential inhibitors to the active site |
Mycobacterium tuberculosis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.6.1.23 |
Mg2+ |
required, binding structure, overview |
Mycobacterium tuberculosis |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.6.1.23 |
dUTP + H2O |
Mycobacterium tuberculosis |
dUTPase is essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis |
dUMP + diphosphate |
- |
? |
|
3.6.1.23 |
dUTP + H2O |
Mycobacterium tuberculosis H37Rv |
dUTPase is essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis |
dUMP + diphosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.6.1.23 |
Mycobacterium tuberculosis |
P9WNS5 |
- |
- |
3.6.1.23 |
Mycobacterium tuberculosis H37Rv |
P9WNS5 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.6.1.23 |
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.6.1.23 |
dUTP + H2O |
dUTPase is essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis |
Mycobacterium tuberculosis |
dUMP + diphosphate |
- |
? |
|
3.6.1.23 |
dUTP + H2O |
development of a robust and continuous dUTPase enzyme activity assay method using phenolred indicator, overview |
Mycobacterium tuberculosis |
dUMP + diphosphate |
- |
? |
|
3.6.1.23 |
dUTP + H2O |
dUTPase is essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis |
Mycobacterium tuberculosis H37Rv |
dUMP + diphosphate |
- |
? |
|
3.6.1.23 |
dUTP + H2O |
development of a robust and continuous dUTPase enzyme activity assay method using phenolred indicator, overview |
Mycobacterium tuberculosis H37Rv |
dUMP + diphosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.6.1.23 |
dUTPase |
- |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.6.1.23 |
25 |
- |
assay at |
Mycobacterium tuberculosis |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
3.6.1.23 |
3.7 |
5.8 |
dUTP |
pH 7.5, 25°C, recombinant wild-type and mutant enzymes |
Mycobacterium tuberculosis |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.6.1.23 |
7.5 |
- |
assay at |
Mycobacterium tuberculosis |