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Literature summary extracted from

  • van der Vlugt-Bergmans, C.J.; van der Werf, M.J.
    Genetic and biochemical characterization of a novel monoterpene epsilon-lactone hydrolase from Rhodococcus erythropolis DCL14 (2001), Appl. Environ. Microbiol., 67, 733-741.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.83
-
 Rhodococcus erythropolis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.83 2,2'-dipyridyl 1 mM, 55% inhibition Rhodococcus erythropolis
3.1.1.83 2-Bromo-4'-nitroacetophenone 1 mM, 84% inhibition Rhodococcus erythropolis
3.1.1.83 CaCl2 1 mM, 27% inhibition Rhodococcus erythropolis
3.1.1.83 CoCl2 1 mM, 71% inhibition Rhodococcus erythropolis
3.1.1.83 EDTA 10 mM, 16% inhibition Rhodococcus erythropolis
3.1.1.83 HgCl2 0.1 mM 95% inhibition Rhodococcus erythropolis
3.1.1.83 MnCl2 1 mM, 37% inhibition Rhodococcus erythropolis
3.1.1.83 p-chloromercuribenzoate 0.1 mM, 26% inhibition Rhodococcus erythropolis
3.1.1.83 phenylhydrazine 1 mM, 17% inhibition Rhodococcus erythropolis
3.1.1.83 SDS 1 mM, 78% inhibition Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.83 35000
-
 gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.83 additional information Rhodococcus erythropolis DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol ?
-
 ?
3.1.1.83 additional information Rhodococcus erythropolis DCL14 DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.83 Rhodococcus erythropolis
-
-
-
3.1.1.83 Rhodococcus erythropolis DCL14
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.83
-
 Rhodococcus erythropolis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.83 culture condition:(4R)-limonene-grown cell
-
 Rhodococcus erythropolis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.83 145
-
-
 Rhodococcus erythropolis

Storage Stability

EC Number Storage Stability Organism
3.1.1.83 -20°C, MLH can be stored for 6 months, without loss of activity Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.83 (4R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + H2O
-
 Rhodococcus erythropolis 6-hydroxy-3-(1-methylethenyl)heptanoic acid
-
 ?
3.1.1.83 (4R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + H2O
-
 Rhodococcus erythropolis DCL14 6-hydroxy-3-(1-methylethenyl)heptanoic acid
-
 ?
3.1.1.83 (6R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + H2O
-
 Rhodococcus erythropolis 5-(hydroxymethyl)-2,6-dimethylhept-6-enoic acid
-
 ?
3.1.1.83 (6R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + H2O
-
 Rhodococcus erythropolis DCL14 5-(hydroxymethyl)-2,6-dimethylhept-6-enoic acid
-
 ?
3.1.1.83 epsilon-caprolactone + H2O
-
 Rhodococcus erythropolis ?
-
 ?
3.1.1.83 epsilon-caprolactone + H2O
-
 Rhodococcus erythropolis DCL14 ?
-
 ?
3.1.1.83 ethyl caproate + H2O 23% of the activity with epsilon-caprolactone Rhodococcus erythropolis ?
-
 ?
3.1.1.83 ethyl caproate + H2O 23% of the activity with epsilon-caprolactone Rhodococcus erythropolis DCL14 ?
-
 ?
3.1.1.83 additional information DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol Rhodococcus erythropolis ?
-
 ?
3.1.1.83 additional information DCL14, catalyzing the ring opening of lactones which are formed during degradation of several monocyclic monoterpenes, including carvone and menthol Rhodococcus erythropolis DCL14 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.83 monomer 1 * 35000, gel filtration Rhodococcus erythropolis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.83 mlhB
-
 Rhodococcus erythropolis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.83 30
-
-
 Rhodococcus erythropolis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.83 13 37 13°C: about 35% of maximal activity, 37°C: 75% of maximal activity Rhodococcus erythropolis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.83 40
-
 inactivation above Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.83 9.5
-
-
 Rhodococcus erythropolis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.83 7.5 10.5 pH 7.5: about 60% of maximal activity, pH 10.5: about 50% of maximal activity Rhodococcus erythropolis