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Literature summary extracted from

  • Van Straaten, K.E.; Hoffort, A.; Palmer, D.R.; Sanders, D.A.
    Purification, crystallization and preliminary X-ray analysis of inositol dehydrogenase (IDH) from Bacillus subtilis (2008), Acta Crystallogr. Sect. F, 64, 98-101.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.18 expression of His-tagged native and selenomethionine-labeled enzyme in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.18 purified recombinant His-tagged native and selenomethionine-labeled enzyme, 12 mg/ml wild-type protein in 25 mM Tris, pH 8.0, and 13.8 mg/ml of selenomethionine-labeled enzyme in 20 mM Tris, pH 8.0, and 5 mM DTT, 4°C, modified microbatch method, equal volumes of protein solution and precipitant solution of 0.001 ml, the latter containing 20%w/v PEG 3350, 0.20 M potassium fluoride, pH 8.5-9.0, are mixed, overlaid with a 1:1 mixture of silicone and paraffin oils, X-ray anomalous diffraction structure determination and analysis at 1.75-2.0 A resolution, molecular replacement Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.18 39170
-
 x * 39170, His-tagged recombinant enzyme, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.18 myo-inositol + NAD+ Bacillus subtilis first enzyme in the catabolic pathway of myo-inositol scyllo-inosose + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.18 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.18 recombinant His-tagged native and selenomethionine-labeled enzyme from Escherichia coli by nickel affinity chromatography to homogeneity Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.18 myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ His176 and Asp172 are proposed to act as the catalytic dyad, in which His176 is proposed to be the acid/base catalyst Bacillus subtilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.18 myo-inositol + NAD+ first enzyme in the catabolic pathway of myo-inositol Bacillus subtilis scyllo-inosose + NADH
-
 r
1.1.1.18 myo-inositol + NAD+ oxidation of the axial hydroxyl group of myo-inositol Bacillus subtilis scyllo-inosose + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.18 ? x * 39170, His-tagged recombinant enzyme, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.18 IDH
-
 Bacillus subtilis
1.1.1.18 inositol dehydrogenase
-
 Bacillus subtilis
1.1.1.18 More IDH is a member of the GFOR/IDH/MocA family Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.18 NAD+
-
 Bacillus subtilis
1.1.1.18 NADH
-
 Bacillus subtilis