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Literature summary extracted from

  • Li, S.Y.; Chen, M.; Li, G.; Yan, Y.L.; Yu, H.Y.; Zhan, Y.H.; Peng, Z.X.; Wang, J.; Lin, M.
    Amino acid substitutions of His296 alter the catalytic properties of Zymomonas mobilis 10232 levansucrase (2008), Acta Biochim. Pol., 55, 201-206.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 expression in Escherichia coli JM109 Zymomonas mobilis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.10 H296C site-directed mutagenesis, no detectable activities Zymomonas mobilis
2.4.1.10 H296E site-directed mutagenesis, no detectable activities Zymomonas mobilis
2.4.1.10 H296K site-directed mutagenesis, minimal hydrolysis and transfructosylation activities, transfructosylation activity is reduced to 4.6% of the wild-type Zymomonas mobilis
2.4.1.10 H296L site-directed mutagenesis, minimal hydrolysis and transfructosylation activities, transfructosylation activity is reduced to 1.0% of the wild-type, mediates the accumulation of low amounts of trisaccharide, but did not produce pentasaccharide Zymomonas mobilis
2.4.1.10 H296Q site-directed mutagenesis, minimal hydrolysis and transfructosylation activities, transfructosylation activity is reduced to 2.5% of the wild-type Zymomonas mobilis
2.4.1.10 H296R site-directed mutagenesis, retains partial hydrolysis and transfructosylation activities, transfructosylation activity is reduced to 23.1% of the wild-type Zymomonas mobilis
2.4.1.10 H296S site-directed mutagenesis, no detectable activities Zymomonas mobilis
2.4.1.10 H296W site-directed mutagenesis, retains partial hydrolysis and transfructosylation activities, transfructosylation activity is reduced to 19.8% of the wild-type Zymomonas mobilis
2.4.1.10 H296Y site-directed mutagenesis, no detectable activities Zymomonas mobilis
2.4.1.10 additional information The His296 mutation influences the substrate recognizing and binding, and then has an effect on sucrose hydrolysis and fructosyl-enzyme intermediate formation. Its mutations weakens the transfructosylation reaction and changes product specificities Zymomonas mobilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Zymomonas mobilis
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 10232 (sacB), expression in Escherichia coli JM109, Zymomonas mobilis levansucrase His296, located in homologous region IV, is equivalent to Bacillus subtilis SacB Arg331 and Gluconacetobacter diazotrophicus LsdA His419
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 Ni-NTA column Zymomonas mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 sucrose + sucrose is assayed in a standard reaction containing 1 ml 10% sucrose and 1 ml enzyme (0.01-0.05mg/ml) incubated at 15°C Zymomonas mobilis D-glucose + levan
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.10 levansucrase
-
 Zymomonas mobilis