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Literature summary extracted from

  • Jurenaite-Urbanaviciene, S.; Serksnaite, J.; Kriukiene, E.; Giedriene, J.; Venclovas, C.; Lubys, A.
    Generation of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains (2007), Proc. Natl. Acad. Sci. USA, 104, 10358-10363.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.21.4 biotechnology generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity Pseudomonas putida
3.1.21.4 biotechnology generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity Thermus scotoductus
3.1.21.4 biotechnology generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity Acinetobacter lwoffii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.4 additional information generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity Pseudomonas putida
3.1.21.4 additional information generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity Thermus scotoductus
3.1.21.4 additional information generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity Acinetobacter lwoffii

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.4 Acinetobacter lwoffii
-
 isoform AloI
-
3.1.21.4 Acinetobacter lwoffii Ks 4-8
-
 isoform AloI
-
3.1.21.4 Pseudomonas putida
-
 isoform PpiI
-
3.1.21.4 Thermus scotoductus
-
 isoform TstI
-

Synonyms

EC Number Synonyms Comment Organism
3.1.21.4 AloI
-
 Acinetobacter lwoffii
3.1.21.4 PPII
-
 Pseudomonas putida
3.1.21.4 TstI
-
 Thermus scotoductus