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Literature summary extracted from
- Protein storage vacuole acidification as a control of storage protein mobilization in soybeans (2007), J. Exp. Bot., 58, 1059-1070.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.21.25 | analysis | subcellular localization of protease C1 and pH conditions in protein storage vacuoles in parenchyma cells of seedling cotyledons and maturing seeds examined | Glycine max |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.25 | vacuole | protein storage vacuoles in parenchyma cells of seedling cotyledons and maturing seeds | Glycine max | 5773 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.25 | 70000 | - |
SDS-PAGE | Glycine max |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.25 | Glycine max | - |
(L.) Merrill | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.25 | gel filtration, SDS-PAGE | Glycine max |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.25 | cotyledon | parenchyma cells of, detected by immunoelectron microscopy | Glycine max | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.25 | additional information | - |
activity during seed germination, activity also determined in two stages of protein storage vacuole development during seed maturation, acidic conditions for activity required, acridine orange used as an indicator of acidic compartments, enzyme activity determined in relation to staining intensities of major storage proteins | Glycine max |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.25 | additional information | subtilisin-like serine protease initiating proteolysis of seed storage proteins during germination and early seedling growth | Glycine max | ? | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.25 | cucumisin | - |
Glycine max |
| 3.4.21.25 | soybean protease C1 | - |
Glycine max |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.25 | 4 | - |
optimum ranges between 3.5 and 4.5 | Glycine max |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.25 | 3.5 | 7 | activity known to decrease precipitously at pH values above the optimum pH range between 3.5 and 4.5, 10-20% activity retained at pH 6 and up to 10% activity retained at pH 7.0 | Glycine max |
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Release 2023.1 (January 2023)
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