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Literature summary extracted from

  • Abrunhosa, L.; Venancio, A.
    Isolation and purification of an enzyme hydrolyzing ochratoxin A from Aspergillus niger (2007), Biotechnol. Lett., 29, 1909-1914.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.17.1 food industry the application of the novel ochratoxin A hydrolytic enzyme to reduce the ochratoxin A contents on some food or feed products is under evaluation Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.1 EDTA 10 mM Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.1 0.5
-
 ochratoxin A
-
 Aspergillus niger
3.4.17.1 1.1
-
 ochratoxin A
-
 Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.1 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.1 the enzyme is purified from the culture medium by acetone precipitation and Macro-Prep High Q anion exchange chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.1 0.09
-
 carboxypeptidase A, pH 5.6 Aspergillus niger
3.4.17.1 2.8
-
 Aspergillus niger extract, pH 5.6 Aspergillus niger
3.4.17.1 2.8
-
 carboxypeptidase A, pH 7.5 Aspergillus niger
3.4.17.1 9.2
-
 Aspergillus niger extract, pH 8.5 Aspergillus niger
3.4.17.1 10.8
-
 carboxypeptidase A, pH 8.5 Aspergillus niger
3.4.17.1 14.4
-
 culture filtrate Aspergillus niger
3.4.17.1 33.5
-
 Aspergillus niger extract, pH 7.5 Aspergillus niger
3.4.17.1 33.5
-
 purification step acetone precipitation Aspergillus niger
3.4.17.1 35.8
-
 purification step Macro-Prep High Q Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.1 ochratoxin A + H2O
-
 Aspergillus niger ochratoxin alpha + L-beta-phenylalanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.1 carboxypeptidase a
-
 Aspergillus niger
3.4.17.1 ochratoxin A hydrolytic enzyme
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.1 37
-
 activity assay Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.1 7.5
-
 activity assay Aspergillus niger