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Literature summary extracted from
- Isolation, heterologous expression and characterization of an endo-polygalacturonase produced by the phytopathogen Burkholderia cepacia (2007), Protein Expr. Purif., 54, 300-308.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.15 | into plasmid pET22b and transformed into Escherichia coli strain DH5alpha, expressed in Escherichia coli BL21(DE3) cells | Burkholderia cepacia |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.15 | periplasm | - |
Burkholderia cepacia | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.15 | 40000 | - |
gel filtration, wild-type | Burkholderia cepacia |
| 3.2.1.15 | 45000 | - |
gel filtration, recombinant protein in solution | Burkholderia cepacia |
| 3.2.1.15 | 47000 | - |
1 * 47000, purified enzyme, SDS-PAGE | Burkholderia cepacia |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.15 | Burkholderia cepacia | P94293 | strain ATCC 25416 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.15 | to homogeneity, 5.8fold by cation exchange chromatography and gel filtration | Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.15 | 3.6 | - |
supernatant | Burkholderia cepacia |
| 3.2.1.15 | 21.1 | - |
5.8fold purified enzyme | Burkholderia cepacia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.15 | polygalacturonic acid + H2O | - |
Burkholderia cepacia | ? | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.15 | monomer | 1 * 47000, purified enzyme, SDS-PAGE | Burkholderia cepacia |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.15 | endo-polygalacturonase | - |
Burkholderia cepacia |
| 3.2.1.15 | endoPG | - |
Burkholderia cepacia |
| 3.2.1.15 | PehA | - |
Burkholderia cepacia |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.15 | additional information | - |
exhibits a relatively flat activity profile in a broad temperature interval, displaying an optimal activity in the temperature interval of 40-55°C | Burkholderia cepacia |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.15 | additional information | - |
the enzyme is thermolabile, being completely inactivated if kept at 50°C for 2 h | Burkholderia cepacia |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.15 | 3.5 | - |
- |
Burkholderia cepacia |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.15 | Burkholderia cepacia | purified enzyme under native conditions | - |
7.8 |
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