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Literature summary extracted from

  • Stillger, T.; Pohl, M.; Wandrey, C.; Liese, A.
    Reaction engineering of benzaldehyde lyase from Pseudomonas fluorescens catalyzing enantioselective C-C bond formation (2006), Org. Proc. Res. Dev., 10, 1172-1177.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.38 0.2
-
 Benzoin in TEA buffer (35 mM, pH 8) containing 0.35 mM thiamine diphosphate, 0.35 mM MgSO4, and 30% (v/v) DMSO, at 20°C Pseudomonas fluorescens
4.1.2.38 2.2
-
 benzaldehyde in TEA buffer (35 mM, pH 8) containing 0.35 mM thiamine diphosphate, 0.35 mM MgSO4, and 30% (v/v) DMSO, at 20°C Pseudomonas fluorescens
4.1.2.38 6
-
 acetaldehyde in TEA buffer (35 mM, pH 8) containing 0.35 mM thiamine diphosphate, 0.35 mM MgSO4, and 30% (v/v) DMSO, at 20°C Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.38 Mg2+ 0.5 mM, substantial for the stability of BAL Pseudomonas fluorescens

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.1.2.38 DMSO BAL is stabilized significantly in the presence of 30% (v/v) DMSO Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.38 Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.38 (R)-benzoin
-
 Pseudomonas fluorescens benzaldehyde + benzaldehyde
-
 r
4.1.2.38 3-chlorobenzaldehyde + acetaldehyde
-
 Pseudomonas fluorescens (R)-(3-chlorophenyl)-2-hydroxy-1-propanone
-
 r
4.1.2.38 benzaldehyde + acetaldehyde
-
 Pseudomonas fluorescens (R)-2-hydroxy-1-phenylpropanone
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.2.38 BAL
-
 Pseudomonas fluorescens
4.1.2.38 benzaldehyde lyase
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.38 9
-
 highest carboligation activity Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.38 7
-
 maximum of enzyme stability is achieved at pH 7 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.38 thiamine diphosphate 0.5 mM, substantial for the stability of BAL Pseudomonas fluorescens