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Literature summary extracted from

  • Fedeli, C.; Carrea, G.; Monti, D.
    On the effects of site-specific mutations on activity and expression of the Streptomyces PMF phospholipase D (2006), J. Mol. Catal. B, 41, 1-7.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.4.4 synthesis production of enzyme in Escherichia coli is strongly influenced by the catalytic activity as cytotoxicity of protein for host is strictly coorelated to enzyme activity. Synthesis of inactive mutants gives recovery yields 5-10 times higher than for wild-type Streptomyces sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4 expression in Escherichia coli. Cytotoxicity of protein for host is strictly coorelated to enzyme activity Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.4 H167N inactive Streptomyces sp.
3.1.4.4 H440N inactive Streptomyces sp.
3.1.4.4 K169S inactive Streptomyces sp.
3.1.4.4 K442S inactive Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.4 extracellular recombinant protein Streptomyces sp.
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Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Streptomyces sp.
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