Literature summary extracted from

Siu, K.K.; Lee, J.E.; Sufrin, J.R.; Moffatt, B.A.; McMillan, M.; Cornell, K.A.; Isom, C.; Howell, P.L.
Molecular determinants of substrate specificity in plant 5-methylthioadenosine nucleosidases (2008), J. Mol. Biol., 378, 112-128.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.4.2.28	additional information	imidazole buffer is the best buffer, whereas phosphate and HEPES buffers result in enzyme velocities that are only 50-60% of those for imidazole	Arabidopsis thaliana

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.4.2.28	into the pET28a+ vector at the NdeI and HindIII sites, overexpression of the enzyme with a cleavable N-terminal His6 tag in Escherichia coli BL21 Codon+ cells	Arabidopsis thaliana
3.2.2.9	expressed in Escherichia coli BL21 Codon+ cells	Arabidopsis thaliana
3.2.2.16	expressed in Escherichia coli BL21 Codon+ cells	Arabidopsis thaliana
3.2.2.30	expressed in Escherichia coli BL21 Codon+ cells	Arabidopsis thaliana

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.4.2.28	MTAN1 in its apo form and in complex with formycin A and 5'-methylthiotubercidin to a resolution of 2.0 A, 1.9 A and 1.8 A, respectively. By hanging-drop, vapor-diffusion technique. Belongs to space group P21. The monomer of MTAN1 has a mixed alpha/beta structure composed of a twisted, ten-stranded mixed beta sheet flanked by seven alpha helices and two short 3(10) helices. In the MTAN1 crystals, the asymmetric unit contains two monomers, which interact via an interface involving the four loops beta2-beta3, beta4-alpha2, beta6-alpha2b and beta8-alpha4, and the alpha2, alpha2b, and alpha5 helices. Both polar and apolar residues are involved in hydrogen bond and van der Waals interactions	Arabidopsis thaliana
2.4.2.28	the intersubunit interface of MTAN is highly hydrophobic and is dominated by van der Waals and hydrophobic interactions	Escherichia coli
3.2.2.16	apo-enzyme as well as in complex with substrate-and transition-state-analogues 5'-methylthiotubercidin and formycin A, at 2.0-1.8 A resolution. Comparison with isoform MTAN2	Arabidopsis thaliana
3.2.2.16	homology model of structure based on isoform MTAN1	Arabidopsis thaliana
3.2.2.16	in complex with 5'-methylthiotubercidin or formycin A, hanging drop vapour diffusion method, in 70 mM sodium acetate trihydrate, pH 4.6, 5.6% (w/v) PEG 4000, 30% (v/v) glycerol	Arabidopsis thaliana

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.4.2.28	F135L	can reduce steric hindrance at the end of the 5'-alkylthio binding subsite, so that longer 5'-substituents may be accommodated more easily in the MTAN2 active site	Arabidopsis thaliana
2.4.2.28	F148L	can reduce steric hindrance at the end of the 5'-alkylthio binding subsite	Arabidopsis thaliana
2.4.2.28	I132V	no significant structural change	Arabidopsis thaliana
2.4.2.28	I145V	no significant structural change	Arabidopsis thaliana
2.4.2.28	L168M	has a more extended side chain, may affect the binding of ligands at the 5'-alkylthio position	Arabidopsis thaliana
2.4.2.28	L181M	has a more extended side chain, may affect the binding of ligands at the 5'-alkylthio position	Arabidopsis thaliana
3.2.2.16	additional information	residues L181/M168 and F148/L135 in isoforms MTAN1 and MTAN2, respectively, are responsible for the divergence in specificity of the isoforms	Arabidopsis thaliana

Inhibitors

EC Number	Inhibitors	Comment	Organism
2.4.2.28	5'-methylthiotubercidin	combination of a more occluded active site in its open state and reduced ligand-induced conformational changes in the plant in contrast to Escherichia coli MTAN	Arabidopsis thaliana
2.4.2.28	5'-methylthiotubercidin	active site of apo-MTAN is more open, and greater conformational changes upon ligand binding in contrast to Arabidopsis MTAN1	Escherichia coli
2.4.2.28	Formycin A	-	Arabidopsis thaliana
2.4.2.28	Formycin A	-	Escherichia coli
2.4.2.28	additional information	Tris buffer severely inhibits the nucleosidase reactions; Tris buffer severely inhibits the nucleosidase reactions	Arabidopsis thaliana
3.2.2.16	5'-methylthiotubercidin	transition state analogue	Arabidopsis thaliana
3.2.2.16	Formycin A	transition state analogue	Arabidopsis thaliana
3.2.2.16	Tris	Tris buffer severely inhibits	Arabidopsis thaliana

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
2.4.2.28	0.0034	-	5'-methylthioadenosine	-	Arabidopsis thaliana
2.4.2.28	0.0071	-	5'-methylthioadenosine	-	Arabidopsis thaliana
3.2.2.9	0.0034	-	5'-methylthioadenosine	in 100 mM sodium phosphate buffer, pH 7.0	Arabidopsis thaliana
3.2.2.16	0.0034	-	5'-methylthioadenosine	22°C	Arabidopsis thaliana
3.2.2.16	0.0071	-	5'-methylthioadenosine	22°C	Arabidopsis thaliana
3.2.2.16	0.0071	-	5'-methylthioadenosine	in 100 mM sodium phosphate buffer, pH 7.0	Arabidopsis thaliana

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.4.2.28	27460	-	sequence analysis	Arabidopsis thaliana
2.4.2.28	28450	-	sequence analysis	Arabidopsis thaliana
3.2.2.9	27462	-	x * 27462, isoform MATN2, calculated from amino acid sequence	Arabidopsis thaliana
3.2.2.30	27462	-	x * 27462, isoform MATN2, calculated from amino acid sequence	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.2.2.9	S-adenosyl-L-homocysteine + H2O	Arabidopsis thaliana	-	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.2.28	Arabidopsis thaliana	Q7XA67	-	-
2.4.2.28	Arabidopsis thaliana	Q9T0I8	-	-
2.4.2.28	Escherichia coli	-	-	-
3.2.2.9	Arabidopsis thaliana	-	-	-
3.2.2.9	Arabidopsis thaliana	Q9T0I8	-	-
3.2.2.16	Arabidopsis thaliana	-	-	-
3.2.2.16	Arabidopsis thaliana	-	isoform MTAN1	-
3.2.2.16	Arabidopsis thaliana	-	isoform MTAN2	-
3.2.2.30	Arabidopsis thaliana	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.4.2.28	by centrifugation and on Ni-NTA column and by gel filtration	Arabidopsis thaliana
2.4.2.28	on Ni-NTA column	Arabidopsis thaliana
3.2.2.9	Ni-NTA agarose column chromatography	Arabidopsis thaliana
3.2.2.16	Ni-NTA agarose column chromatography	Arabidopsis thaliana
3.2.2.16	Ni-NTA agarose column chromatography and Superdex-200 gel filtration	Arabidopsis thaliana
3.2.2.30	Ni-NTA agarose column chromatography	Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.4.2.28	0.38	-	with adenosine as substrate	Arabidopsis thaliana
2.4.2.28	0.46	-	with 5'-choroadenosine as substrate	Arabidopsis thaliana
2.4.2.28	0.83	-	with 5'-choroadenosine as substrate	Arabidopsis thaliana
2.4.2.28	0.96	-	with S-adenosylhomocysteine as substrate	Arabidopsis thaliana
2.4.2.28	1.06	-	with 5'-iodoadenosine as substrate	Arabidopsis thaliana
2.4.2.28	1.36	-	with 5'-phenylthioadenosine as substrate	Arabidopsis thaliana
2.4.2.28	1.5	-	with 5'-butylthioadenosine as substrate	Arabidopsis thaliana
2.4.2.28	1.85	-	with 5'-isobutylthioadenosine as substrate	Arabidopsis thaliana
2.4.2.28	1.98	-	with 5'-isobutylthioadenosine as substrate	Arabidopsis thaliana
2.4.2.28	2.44	-	with 5'-phenylthioadenosine as substrate	Arabidopsis thaliana
2.4.2.28	2.6	-	with 5'-ethylthioadenosine as substrate	Arabidopsis thaliana
2.4.2.28	2.72	-	with 5'-butylthioadenosine as substrate	Arabidopsis thaliana
2.4.2.28	3.14	-	with 5'-iodoadenosine as substrate	Arabidopsis thaliana
2.4.2.28	4.42	-	with 5'-ethylthioadenosine as substrate	Arabidopsis thaliana
2.4.2.28	6.25	-	with 5'-methylthioadenosine as substrate	Arabidopsis thaliana
2.4.2.28	11.5	-	with 5'-methylthioadenosine as substrate	Arabidopsis thaliana
3.2.2.9	0.9	-	using S-adenosyl-L-homocysteine as substrate, at pH 6.0 and 22°C	Arabidopsis thaliana
3.2.2.16	0.38	-	substrate adenosine, 22°C	Arabidopsis thaliana
3.2.2.16	0.46	-	substrate 5'-chloroadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	0.83	-	substrate 5'-chloroadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	0.9	-	substrate S-adenosylhomocysteine, 22°C	Arabidopsis thaliana
3.2.2.16	1.06	-	substrate 5'-iodoadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	1.36	-	substrate 5'-phenylthioadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	1.5	-	substrate 5'-butylthioadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	1.85	-	substrate 5'-isobutylthioadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	1.98	-	substrate 5'-isobutylthioadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	2.44	-	substrate 5'-phenylthioadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	2.6	-	substrate 5'-ethylthioadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	2.72	-	substrate 5'-butylthioadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	3.14	-	substrate 5'-iodoadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	4.42	-	substrate 5'-ethylthioadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	6.25	-	substrate 5'-methylthioadenosine, 22°C	Arabidopsis thaliana
3.2.2.16	11.05	-	substrate 5'-methylthioadenosine, 22°C	Arabidopsis thaliana
3.2.2.30	0.38	-	using adenosine as substrate, at pH 6.0 and 22°C	Arabidopsis thaliana
3.2.2.30	0.83	-	using 5'-chloroadenosine as substrate, at pH 6.0 and 22°C	Arabidopsis thaliana
3.2.2.30	1.06	-	using 5'-iodoadenosine as substrate, at pH 6.0 and 22°C	Arabidopsis thaliana
3.2.2.30	1.5	-	using 5'-butylthioadenosine as substrate, at pH 6.0 and 22°C	Arabidopsis thaliana
3.2.2.30	1.98	-	using 5'-isobutylthioadenosine as substrate, at pH 6.0 and 22°C	Arabidopsis thaliana
3.2.2.30	2.44	-	using 5'-phenylthioadenosine as substrate, at pH 6.0 and 22°C	Arabidopsis thaliana
3.2.2.30	6.25	-	using 5'-methylthioadenosine as substrate, at pH 6.0 and 22°C	Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.4.2.28	5'-butylthioadenosine + phosphate	24% relative activity	Arabidopsis thaliana	adenine + 5-butylthio-D-ribose 1-phosphate	-	?
2.4.2.28	5'-butylthioadenosine + phosphate	25% relative activity	Arabidopsis thaliana	adenine + 5-butylthio-D-ribose 1-phosphate	-	?
2.4.2.28	5'-chloroadenosine + phosphate	13% relative activity	Arabidopsis thaliana	adenine + 5-deoxy-5-chloro-D-ribose 1-phosphate	-	?
2.4.2.28	5'-chloroadenosine + phosphate	4% relative activity	Arabidopsis thaliana	adenine + 5-deoxy-5-chloro-D-ribose 1-phosphate	-	?
2.4.2.28	5'-ethylthioadenosine + phosphate	40% relative activity	Arabidopsis thaliana	adenine + 5-ethylthio-D-ribose 1-phosphate	-	?
2.4.2.28	5'-ethylthioadenosine + phosphate	42% relative activity	Arabidopsis thaliana	adenine + 5-ethylthio-D-ribose 1-phosphate	-	?
2.4.2.28	5'-iodoadenosine + phosphate	17% relative activity	Arabidopsis thaliana	adenine + 5-deoxy-5-iodo-D-ribose 1-phosphate	-	?
2.4.2.28	5'-iodoadenosine + phosphate	28% relative activity	Arabidopsis thaliana	adenine + 5-deoxy-5-iodo-D-ribose 1-phosphate	-	?
2.4.2.28	5'-isobutylthioadenosine + phosphate	32% relative activity	Arabidopsis thaliana	adenine + 5-isobutylthio-D-ribose 1-phosphate	-	?
2.4.2.28	5'-isobutylthioadenosine + phosphate	17% relative activity	Arabidopsis thaliana	adenine + 5-isobutylthio-D-ribose 1-phosphate	-	?
2.4.2.28	5'-methylthioadenosine + phosphate	100% relative activity	Arabidopsis thaliana	adenine + 5-methylthio-D-ribose 1-phosphate	-	?
2.4.2.28	5'-phenylthioadenosine + phosphate	39% relative activity	Arabidopsis thaliana	adenine + 5-phenylthio-D-ribose 1-phosphate	-	?
2.4.2.28	5'-phenylthioadenosine + phosphate	12% relative activity	Arabidopsis thaliana	adenine + 5-phenylthio-D-ribose 1-phosphate	-	?
2.4.2.28	adenosine + phosphate	6% relative activity	Arabidopsis thaliana	adenine + D-ribose 1-phosphate	-	?
2.4.2.28	additional information	the plant active site is likely less flexible, which may contribute to its reduced efficiency in binding larger substrates. The 5'-alkylthio binding subsite is more constricted in MTAN1	Arabidopsis thaliana	?	-	?
2.4.2.28	S-adenosylhomocysteine + phosphate	14% relative activity	Arabidopsis thaliana	?	-	?
3.2.2.9	5'-butylthioadenosine + H2O	24% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-butylthio-D-ribose	-	?
3.2.2.9	5'-chloroadenosine + H2O	13% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-chloro-D-ribose	-	?
3.2.2.9	5'-ethylthioadenosine + H2O	42% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-ethylthio-D-ribose	-	?
3.2.2.9	5'-iodoadenosine + H2O	17% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-iodo-D-ribose	-	?
3.2.2.9	5'-isobutylthioadenosine + H2O	32% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-isobutylthio-D-ribose	-	?
3.2.2.9	5'-methylthioadenosine + H2O	100% activity	Arabidopsis thaliana	5-methylthio-D-ribose + adenine	-	?
3.2.2.9	5'-phenylthioadenosine + H2O	39% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-phenylthio-D-ribose	-	?
3.2.2.9	adenosine + H2O	6% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + D-ribose	-	?
3.2.2.9	S-adenosyl-L-homocysteine + H2O	14% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + S-ribosyl-L-homocysteine	-	?
3.2.2.9	S-adenosyl-L-homocysteine + H2O	-	Arabidopsis thaliana	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine	-	?
3.2.2.16	5'-butylthioadenosine + H2O	-	Arabidopsis thaliana	5-butylthio-D-ribose + adenine	-	?
3.2.2.16	5'-butylthioadenosine + H2O	25% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-butylthio-D-ribose	-	?
3.2.2.16	5'-chloroadenosine + H2O	-	Arabidopsis thaliana	5-chloro-D-ribose + adenine	-	?
3.2.2.16	5'-chloroadenosine + H2O	4% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-chloro-D-ribose	-	?
3.2.2.16	5'-ethylthioadenosine + H2O	-	Arabidopsis thaliana	5-ethylthio-D-ribose + adenine	-	?
3.2.2.16	5'-ethylthioadenosine + H2O	40% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-ethylthio-D-ribose	-	?
3.2.2.16	5'-iodoadenosine + H2O	28% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-iodo-D-ribose	-	?
3.2.2.16	5'-iodothioadenosine + H2O	-	Arabidopsis thaliana	5-iodothio-D-ribose + adenine	-	?
3.2.2.16	5'-isobutylthioadenosine + H2O	-	Arabidopsis thaliana	5-isobutylthioribose + adenine	-	?
3.2.2.16	5'-isobutylthioadenosine + H2O	17% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-isobutylthio-D-ribose	-	?
3.2.2.16	5'-methylthioadenosine + H2O	-	Arabidopsis thaliana	5-methylthio-D-ribose + adenine	-	?
3.2.2.16	5'-methylthioadenosine + H2O	100% activity	Arabidopsis thaliana	5-methylthio-D-ribose + adenine	-	?
3.2.2.16	5'-phenylthioadenosine + H2O	-	Arabidopsis thaliana	5-phenylthio-D-ribose + adenine	-	?
3.2.2.16	5'-phenylthioadenosine + H2O	12% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + 5-phenylthio-D-ribose	-	?
3.2.2.16	adenosine + H2O	-	Arabidopsis thaliana	adenine + D-ribose	-	?
3.2.2.16	additional information	no substrate: S-adenosylhomocysteine, adenosine	Arabidopsis thaliana	?	-	?
3.2.2.16	additional information	no activity towards S-adenosyl-L-homocysteine and adenosine	Arabidopsis thaliana	?	-	?
3.2.2.16	S-adenosyl-L-homocysteine + H2O	14% activity compared to 5'-methylthioadenosine	Arabidopsis thaliana	adenine + S-ribosyl-L-homocysteine	-	?
3.2.2.16	S-adenosylhomocysteine + H2O	-	Arabidopsis thaliana	adenine + S-ribosylhomocysteine	-	?
3.2.2.30	5'-butylthioadenosine + H2O	-	Arabidopsis thaliana	?	-	?
3.2.2.30	5'-chloroadenosine + H2O	-	Arabidopsis thaliana	?	-	?
3.2.2.30	5'-iodoadenosine + H2O	-	Arabidopsis thaliana	?	-	?
3.2.2.30	5'-isobutylthioadenosine + H2O	-	Arabidopsis thaliana	?	-	?
3.2.2.30	5'-methylthioadenosine + H2O	-	Arabidopsis thaliana	?	-	?
3.2.2.30	5'-phenylthioadenosine + H2O	-	Arabidopsis thaliana	?	-	?
3.2.2.30	adenosine + H2O	-	Arabidopsis thaliana	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.4.2.28	dimer	crystallography	Escherichia coli
2.4.2.28	dimer	crystallography	Arabidopsis thaliana
3.2.2.9	?	x * 27462, isoform MATN2, calculated from amino acid sequence	Arabidopsis thaliana
3.2.2.16	dimer	x-ray crystallography	Arabidopsis thaliana
3.2.2.30	?	x * 27462, isoform MATN2, calculated from amino acid sequence	Arabidopsis thaliana

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.2.28	5'-methylthioadenosine nucleosidase	-	Escherichia coli
2.4.2.28	5'-methylthioadenosine nucleosidase	-	Arabidopsis thaliana
2.4.2.28	MTAN	-	Escherichia coli
2.4.2.28	MTAN1	-	Arabidopsis thaliana
2.4.2.28	MTAN2	-	Arabidopsis thaliana
2.4.2.28	MTN1	-	Arabidopsis thaliana
2.4.2.28	MTN2	-	Arabidopsis thaliana
3.2.2.9	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase	-	Arabidopsis thaliana
3.2.2.9	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2	-	Arabidopsis thaliana
3.2.2.9	MTA/SAH nucleosidase	-	Arabidopsis thaliana
3.2.2.9	MTAN2	-	Arabidopsis thaliana
3.2.2.9	MTAN2	isoform	Arabidopsis thaliana
3.2.2.16	5'-methylthioadenosine nucleosidase	-	Arabidopsis thaliana
3.2.2.16	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase	-	Arabidopsis thaliana
3.2.2.16	MTA nucleosidase	-	Arabidopsis thaliana
3.2.2.16	MTA/SAH nucleosidase	-	Arabidopsis thaliana
3.2.2.16	MTAN1	-	Arabidopsis thaliana
3.2.2.16	MTAN2	-	Arabidopsis thaliana
3.2.2.30	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2	-	Arabidopsis thaliana
3.2.2.30	MTAN2	isoform	Arabidopsis thaliana

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
2.4.2.28	2	-	5'-methylthioadenosine	-	Arabidopsis thaliana
2.4.2.28	18.7	-	5'-methylthioadenosine	-	Arabidopsis thaliana
3.2.2.9	2	-	5'-methylthioadenosine	in 100 mM sodium phosphate buffer, pH 7.0	Arabidopsis thaliana
3.2.2.16	2	-	5'-methylthioadenosine	22°C	Arabidopsis thaliana
3.2.2.16	18.7	-	5'-methylthioadenosine	22°C	Arabidopsis thaliana
3.2.2.16	18.7	-	5'-methylthioadenosine	in 100 mM sodium phosphate buffer, pH 7.0	Arabidopsis thaliana

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.4.2.28	6	-	-	Arabidopsis thaliana
2.4.2.28	8	-	-	Arabidopsis thaliana
3.2.2.9	6	-	-	Arabidopsis thaliana
3.2.2.16	6	-	-	Arabidopsis thaliana
3.2.2.16	8	-	-	Arabidopsis thaliana
3.2.2.30	6	-	-	Arabidopsis thaliana

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.4.2.28	0.0083	-	5'-methylthiotubercidin	at 0.005 mM	Arabidopsis thaliana
2.4.2.28	0.048	-	Formycin A	at 0.05 mM	Arabidopsis thaliana

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.4.2.28	588	-	5'-methylthioadenosine	-	Arabidopsis thaliana
2.4.2.28	2600	-	5'-methylthioadenosine	-	Arabidopsis thaliana