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Literature summary extracted from

  • Ding, L.; Hofius, D.; Hajirezaei, M.R.; Fernie, A.R.; Boernke, F.; Sonnewald, U.
    Functional analysis of the essential bifunctional tobacco enzyme 3-dehydroquinate dehydratase/shikimate dehydrogenase in transgenic tobacco plants (2007), J. Exp. Bot., 58, 2053-2067.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 DNA and amino acid sequence determination and analysis Nicotiana tabacum
1.1.1.25 DNA and amino acid sequence determination and analysis, expression of Hs6-tagged isozyme 1 in Escherichia coli, transfection of Nicotina plants Nicotiana tabacum
1.1.1.25 expression in Escherichia coli Nicotiana tabacum
4.2.1.10 DNA and amino acid sequence determination and analysis Nicotiana tabacum
4.2.1.10 NtDHD/SHD-1 is expressed in Escherichia coli Nicotiana tabacum
4.2.1.10 NtDHD/SHD-2 Nicotiana tabacum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.25 additional information cytosolic isoform cannot complement loss of the plastidial isoform Nicotiana tabacum
1.1.1.25 additional information expression of endogenous DHD/SHD-1 is suppressed by RNAi in transgenic tobacco plants, the transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin, but accumulation of dehydroquinate and shikimate, possibly due to existence of a parallel extra-plastidic shikimate pathway into which dehydroquinate is diverted with a second gene DHD/SHD-2 in tobacco lacking a plastidic targeting sequence, the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway, phenotype, overview Nicotiana tabacum
1.1.1.25 additional information suppression of shikimate dehydrogenase activity by RNAi. Transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin. Dehydroquinate, the substrate of the enzyme, accumulates, and due to a second, cytosolic enzyme, the product, shikimate accumulates Nicotiana tabacum
4.2.1.10 additional information expression of endogenous DHD/SHD-1 is suppressed by RNAi in transgenic tobacco plants, the transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin, but accumulation of dehydroquinate and shikimate, possibly due to existence of a parallel extra-plastidic shikimate pathway into which dehydroquinate is diverted with a second gene DHD/SHD-2 in tobacco lacking a plastidic targeting sequence, the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway, phenotype, overview Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.10 additional information RNA interference of NtDHD/SHD-1 strongly inhibits DHD/SHD activity in transgenic tobacco plants Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.25 0.031
-
 NADP+ 22°C, pH 9.0 Nicotiana tabacum
1.1.1.25 0.13
-
 shikimate 22°C, pH 9.0 Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.25 chloroplast DHD/SHD-1 containing a plastidic targeting sequence Nicotiana tabacum 9507
-
1.1.1.25 cytosol DHD/SHD-2 Nicotiana tabacum 5829
-
1.1.1.25 cytosol exclusively cytosolic Nicotiana tabacum 5829
-
1.1.1.25 plastid
-
 Nicotiana tabacum 9536
-
4.2.1.10 cytosol NtDHD/SHD-2 is the cytosolic isoenzyme Nicotiana tabacum 5829
-
4.2.1.10 cytosol DHD/SHD-2 Nicotiana tabacum 5829
-
4.2.1.10 plastid
-
 Nicotiana tabacum 9536
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.25 L-quinate + NADP+ Nicotiana tabacum
-
 3-dehydroquinate + NADPH + H+
-
 r
1.1.1.25 additional information Nicotiana tabacum the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway ?
-
 ?
1.1.1.25 shikimate + NAD+ Nicotiana tabacum
-
 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.25 shikimate + NADP+ Nicotiana tabacum
-
 3-dehydroshikimate + NADPH + H+
-
 r
4.2.1.10 dehydroquinate Nicotiana tabacum
-
 dehydroshikimate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Nicotiana tabacum
-
-
-
1.1.1.25 Nicotiana tabacum Q6PUF9 DHD/SHD-1; plastidic isozyme
-
1.1.1.25 Nicotiana tabacum Q6PUG0 cytosolic isoform
-
1.1.1.25 Nicotiana tabacum Q6PUG0 DHD/SHD-2; cytosolic isozyme
-
4.2.1.10 Nicotiana tabacum Q6PUF9 cv. Samsun NN
-
4.2.1.10 Nicotiana tabacum Q6PUG0 cv. Samsun NN
-
4.2.1.10 Nicotiana tabacum Q6PUG0 DHD/SHD-2; cytosolic isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.25 recombinant protein Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.25 leaf
-
 Nicotiana tabacum
-
4.2.1.10 leaf
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 3-dehydroshikimate + NADPH
-
 Nicotiana tabacum shikimate + NADP+
-
 ?
1.1.1.25 L-quinate + NADP+
-
 Nicotiana tabacum 3-dehydroquinate + NADPH + H+
-
 r
1.1.1.25 additional information the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway Nicotiana tabacum ?
-
 ?
1.1.1.25 additional information the enzyme also shows 3-dehydroquinate dehydratase activity, EC 4.2.1.10 Nicotiana tabacum ?
-
 ?
1.1.1.25 shikimate + NAD+
-
 Nicotiana tabacum 3-dehydroshikimate + NADH + H+
-
 r
1.1.1.25 shikimate + NADP+
-
 Nicotiana tabacum 3-dehydroshikimate + NADPH + H+
-
 ?
1.1.1.25 shikimate + NADP+
-
 Nicotiana tabacum 3-dehydroshikimate + NADPH + H+
-
 r
4.2.1.10 dehydroquinate
-
 Nicotiana tabacum dehydroshikimate + H2O
-
 r
4.2.1.10 additional information the enzyme also shows shikimate dehydrogenase activity, EC 1.1.1.25 Nicotiana tabacum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.25 3-dehydroquinate dehydratase/shikimate dehydrogenase
-
 Nicotiana tabacum
1.1.1.25 DHD/SHD
-
 Nicotiana tabacum
1.1.1.25 More cf. EC 1.1.1.25 Nicotiana tabacum
4.2.1.10 3-dehydroquinate dehydratase /shikimate dehydrogenase isoform 1
-
 Nicotiana tabacum
4.2.1.10 3-dehydroquinate dehydratase /shikimate dehydrogenase isoform 2
-
 Nicotiana tabacum
4.2.1.10 3-dehydroquinate dehydratase/shikimate dehydrogenase
-
 Nicotiana tabacum
4.2.1.10 DHD/SHD
-
 Nicotiana tabacum
4.2.1.10 More cf. EC 1.1.1.25 Nicotiana tabacum
4.2.1.10 NtDHD/SHD-1
-
 Nicotiana tabacum
4.2.1.10 NtDHD/SHD-2 cytosolic isoenzyme Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.25 22
-
 assay at room temperature Nicotiana tabacum
1.1.1.25 30 45
-
 Nicotiana tabacum
4.2.1.10 22
-
 assay at room temperature Nicotiana tabacum
4.2.1.10 30 45
-
 Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.25 6.8 7.2 formation of shikimate Nicotiana tabacum
1.1.1.25 9 9.4 formation of 3-dehydroshikimate Nicotiana tabacum
1.1.1.25 9
-
 assay at Nicotiana tabacum
4.2.1.10 9
-
 assay at Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 NADP+
-
 Nicotiana tabacum
1.1.1.25 NADPH
-
 Nicotiana tabacum
4.2.1.10 NAD+
-
 Nicotiana tabacum
4.2.1.10 NADH
-
 Nicotiana tabacum