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Literature summary extracted from

  • Bellostas, N.; Sörensen, J.C.; Sörensen, H.
    Micellar electrokinetic capillary chromatography - synchronous monitoring of substrate and products in the myrosinase catalysed hydrolysis of glucosinolates (2006), J. Chromatogr. A, 1130, 246-252.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.147 analysis micellar electrokinetic capillary chromatography method for monitoring the myrosinase catalyzed hydrolysis of 2-hydroxy substituted glucosinolates and formation of the corresponding oxazolidine-2-thiones and nitriles. Method has a detection limit of 0.04 mM and a limit of quantification of 0.2 mM and 0.3 mM for the glucosibarin-derived oxazolidine-2-thione and nitrile, respectivityl Brassica carinata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Brassica carinata
-
-
-
3.2.1.147 Brassica carinata BRK-147-A
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 (2R)-2-hydroxy-2-phenylethylglucosinolate + H2O i.e. glucosibarin Brassica carinata D-glucose + ?
-
?
3.2.1.147 (2R)-2-hydroxy-2-phenylethylglucosinolate + H2O i.e. glucosibarin Brassica carinata BRK-147-A D-glucose + ?
-
?
3.2.1.147 gluconasturtiin + H2O
-
Brassica carinata D-glucose + ?
-
?
3.2.1.147 gluconasturtiin + H2O
-
Brassica carinata BRK-147-A D-glucose + ?
-
?
3.2.1.147 glucotropaeolin + H2O
-
Brassica carinata D-glucose + ?
-
?
3.2.1.147 glucotropaeolin + H2O
-
Brassica carinata BRK-147-A D-glucose + ?
-
?