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Literature summary extracted from

  • Cho, J.; Choi, K.; Darden, T.; Reynolds, P.R.; Petitte, J.N.; Shears, S.B.
    Avian multiple inositol polyphosphate phosphatase is an active phytase that can be engineered to help ameliorate the planets "phosphate crisis" (2006), J. Biotechnol., 126, 248-259.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.3.62 biotechnology recombinant avian MINPP (0.7 micromol/mg protein/min) is the most active phytase found to date in any animal cell Gallus gallus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.62 cDNA encoding residues 19-445 of avian MINPP or residues 31-483 of human MINPP lacking the N-terminal ER targeting sequences and the C-terminal ER retention signals are amplified by PCR and cloned into pGEM Tvector and transformed into Escherichia coli Gallus gallus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.62 Q78A Km: 0.06 (myo-inositol hexakisphosphate). Q78A mutant shows differences in its specificity profile compared to wild-type enzyme: relative to inositol hexakisphosphate, the activity towards other organic phosphates are as follows: p-nitrophenyl phosphate: 7%, ATP: 12%, ribose 1-phosphate: 3% Gallus gallus
3.1.3.62 T27G Km: 0.092 (myo-inositol hexakisphosphate) Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.62 0.06
-
 myo-inositol hexakisphosphate mutant Q78A Gallus gallus
3.1.3.62 0.092
-
 myo-inositol hexakisphosphate mutant T27G Gallus gallus
3.1.3.62 0.14
-
 myo-inositol hexakisphosphate
-
 Gallus gallus
3.1.3.62 1.74
-
 scyllo-inositol hexakisphosphate
-
 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.62 64000
-
 SDS-PAGE Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.62 Gallus gallus
-
 avian
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.62 purification is carried out on a Ni-sepharose column Gallus gallus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.62 additional information
-
 activity of MINPP towards other phosphates, relative to inositol hexakisphosphate, is as follows: p-nitrophenyl phosphate: 17%, ATP: 11%, D-ribose 1-phosphate: 0.31% Gallus gallus
3.1.3.62 additional information
-
 Q78A mutant shows differences in its specificity profile compared to wild-type enzyme: relative to inositol hexakisphosphate, the activity towards other phosphates are as follows: p-nitrophenyl phosphate: 7%, ATP: 12%, D-ribose 1-phosphate: 3% Gallus gallus
3.1.3.62 0.7
-
 specific activity of recombinant avian MINPP Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.62 myo-inositol hexakisphosphate + H2O
-
 Gallus gallus myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.62 scyllo-inositol hexakisphosphate + H2O
-
 Gallus gallus scyllo-inositol pentakisphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.62 Minpp
-
 Gallus gallus
3.1.3.62 multiple inositol polyphosphate phosphatase
-
 Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.62 40
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.62 5
-
 assay at Gallus gallus