EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.19 | AMP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
4.3.1.19 | CMP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.1.19 | into the pRSETC vector for expression in Escherichia coli BL21DE3 pLysS cells | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.1.19 | data for two crystal forms are collected to resolutions of 2.2 and 1.7 A, crystals obtainted in the presence of CMP diffract to a resolution of 3-3.5 A, in the presence of AMP poorly | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.19 | 16 | - |
L-threonine | in the presence of 5 mM AMP | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.3.1.19 | 32 | - |
L-threonine | in the presence of 5 mM CMP | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.3.1.19 | 123 | - |
L-threonine | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.3.1.19 | 45000 | - |
determined by gel-filtration in the absence of CMP and AMP | Salmonella enterica subsp. enterica serovar Typhimurium |
4.3.1.19 | 100000 | - |
determined by gel-filtration in the presence of CMP | Salmonella enterica subsp. enterica serovar Typhimurium |
4.3.1.19 | 120000 | - |
determined by gel-filtration in the presence of AMP | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.19 | L-threonine | Salmonella enterica subsp. enterica serovar Typhimurium | - |
2-oxobutanoate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.19 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.1.19 | using a nickel-nitrilotriacetic acid affinity column | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.19 | L-threonine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxobutanoate + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.1.19 | dimer | native structure | Salmonella enterica subsp. enterica serovar Typhimurium |
4.3.1.19 | tetramer | in the complex with CMP | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.1.19 | Threonine deaminase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.1.19 | 25 | - |
activity assay | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.19 | 8 | - |
activity assay | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.19 | pyridoxal 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |