Literature summary extracted from
Versees, W.; Spaepen, S.; Vanderleyden, J.; Steyaert, J.
The crystal structure of phenylpyruvate decarboxylase from Azospirillum brasilense at 1.5 A resolution. Implications for its catalytic and regulatory mechanism (2007), FEBS J., 274, 2363-2375.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.1.1.43 |
expressed in Escherichia coli Bl21-CodonPlus (DE3)-RP cells |
Azospirillum brasilense |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.1.1.43 |
hanging drop vapour diffusion method with precipitant solution containing 15% PEG4000 (w /v), 10% glycerol (v /v) in 100 mM HEPES buffer pH 7.5, at 20°C |
Azospirillum brasilense |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.1.1.43 |
Mg2+ |
required, PPDC binds two molecules of Mg2+ per dimer |
Azospirillum brasilense |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.1.1.43 |
58000 |
- |
4 * 58000, X-ray crystallography, asymmetric dimer of dimers, in the asymmetric unit two subunits of PPDC are tightly packed to form a dimer related by noncrystallographic two-fold symmetry |
Azospirillum brasilense |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.1.43 |
Azospirillum brasilense |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.1.1.43 |
Ni-nitrilotriacetic acid affinity chromatography and Superdex-200 gel filtration |
Azospirillum brasilense |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.1.43 |
indolepyruvate |
higher affinity for indolepyruvate than for phenylpyruvate but a 10fold higher specificity for the latter |
Azospirillum brasilense |
indoleacetaldehyde + CO2 |
- |
? |
|
4.1.1.43 |
additional information |
completely inactive toward benzoylformate |
Azospirillum brasilense |
? |
- |
? |
|
4.1.1.43 |
Phenylpyruvate |
converts phenylpyruvate with a 10fold higher specificity than indolepyruvate despite a slightly higher affinity for the latter |
Azospirillum brasilense |
Phenylacetaldehyde + CO2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.1.1.43 |
homotetramer |
4 * 58000, X-ray crystallography, asymmetric dimer of dimers, in the asymmetric unit two subunits of PPDC are tightly packed to form a dimer related by noncrystallographic two-fold symmetry |
Azospirillum brasilense |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.1.1.43 |
phenyl/indolepyruvate decarboxylase |
- |
Azospirillum brasilense |
4.1.1.43 |
PPDC |
- |
Azospirillum brasilense |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.1.1.43 |
thiamine diphosphate |
dependent, PPDC binds two molecules of thiamine diphosphate per dimer |
Azospirillum brasilense |
|