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Literature summary extracted from

  • Sorensen, H.R.; Pedersen, S.; Jorgensen, C.T.; Meyer, A.S.
    Enzymatic hydrolysis of wheat arabinoxylan by a recombinant "minimal" enzyme cocktail containing beta-xylosidase and novel endo-1,4-beta-xylanase and alpha-L-arabinofuranosidase activities (2007), Biotechnol. Prog., 23, 100-107.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.37 degradation hydrolysis of water-soluble and water-insoluble arabinoxylan and whole vinasse by an enzyme cocktail containing a 20%:20%:20%:40% mixture and a 25%:25%:25%:25% mixture, respectively, of the GH43 alpha-L-arabinofuranosidase from Humicola insolens, the GH51 alpha-L-arabinofuranosidase from M. giganteus, a GH10 endo-1,4-beta-xylanase from H. insolens, and a GH3 beta-xylosidase from Trichoderma reesei. The optimal dosages of the minimal enzyme cocktails are 0.4, 0.3, and 0.2 g enzyme protein per kilogram of substrate dry matter for the water-soluble wheat arabinoxylan, the water-insoluble wheat arabinoxylan, and the vinasse, respectively Trichoderma reesei
3.2.1.55 degradation enzymatic hydrolysis of wheat arabinoxylan. Treatment of water-soluble and water-insoluble wheat arabinoxylan with an enzyme cocktail containing a 20%:20%:20%:40% mixture and a 25%:25%:25%:25% mixture, respectively, of the GH43 alpha-L-arabinofuranosidase from Humicola insolens (Abf II), the GH51 alpha-L-arabinofuranosidase from Meripilus giganteus (Abf III), a GH10 endo-1,4-beta-xylanase from Humicola insolens (Xyl III), and a GH3 beta-xylosidase from Trichoderma reesei releases 322 mg of arabinose and 512 mg of xylose per gram of water-soluble wheat arabinoxylan dry matter and 150 mg of arabinose and 266 mg of xylose per gram of water-insoluble wheat arabinoxylan dry matter after 24 h at pH 5, 50°C. A 10%:40%:50% mixture of Abf II, Abf III, and beta-xyl releases 56 mg of arabinose and 91 mg of xylose per gram of vinasse dry matter after 24 h at pH 5, 50°C. The optimal dosages of the enzyme cocktails are determined to be 0.4, 0.3, and 0.2 g enzyme protein per kilogram of substrate dry matter for the water-soluble wheat arabinoxylan, the water-insoluble wheat arabinoxylan, and the vinasse, respectively Humicola insolens
3.2.1.55 degradation enzymatic hydrolysis of wheat arabinoxylan. Treatment of water-soluble and water-insoluble wheat arabinoxylan with an enzyme cocktail containing a 20%:20%:20%:40% mixture and a 25%:25%:25%:25% mixture, respectively, of the GH43 alpha-L-arabinofuranosidase from Humicola insolens (Abf II), the GH51 alpha-L-arabinofuranosidase from Meripilus giganteus (Abf III), a GH10 endo-1,4-beta-xylanase from Humicola insolens (Xyl III), and a GH3 beta-xylosidase from Trichoderma reesei releases 322 mg of arabinose and 512 mg of xylose per gram of water-soluble wheat arabinoxylan dry matter and 150 mg of arabinose and 266 mg of xylose per gram of water-insoluble wheat arabinoxylan dry matter after 24 h at pH 5, 50°C. A 10%:40%:50% mixture of Abf II, Abf III, and beta-xyl releases 56 mg of arabinose and 91 mg of xylose per gram of vinasse dry matter after 24 h at pH 5, 50°C. The optimal dosages of the enzyme cocktails are determined to be 0.4, 0.3, and 0.2 g enzyme protein per kilogram of substrate dry matter for the water-soluble wheat arabinoxylan, the water-insoluble wheat arabinoxylan, and the vinasse, respectively Meripilus giganteus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Trichoderma reesei Q92458
-
-
3.2.1.55 Humicola insolens
-
-
-
3.2.1.55 Meripilus giganteus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 vinasse + H2O
-
 Trichoderma reesei D-xylose + ? hydrolysis by an enzyme cocktail containing a 20%:20%:20%:40% mixture and a 25%:25%:25%:25% mixture, respectively, of the GH43 alpha-L-arabinofuranosidase from Humicola insolens, the GH51 alpha-L-arabinofuranosidase from M. giganteus, a GH10 endo-1,4-beta-xylanase from H. insolens, and a GH3 beta-xylosidase from Trichoderma reesei ?
3.2.1.37 wheat arabinoxylan + H2O
-
 Trichoderma reesei D-xylose + ? hydrolysis by an enzyme cocktail containing a 20%:20%:20%:40% mixture and a 25%:25%:25%:25% mixture, respectively, of the GH43 alpha-L-arabinofuranosidase from Humicola insolens, the GH51 alpha-L-arabinofuranosidase from M. giganteus, a GH10 endo-1,4-beta-xylanase from H. insolens, and a GH3 beta-xylosidase from Trichoderma reesei ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 Abf II
-
 Humicola insolens
3.2.1.55 Abf III
-
 Meripilus giganteus
3.2.1.55 alpha-L-arabinofuranosidase II
-
 Humicola insolens
3.2.1.55 alpha-L-arabinofuranosidase III
-
 Meripilus giganteus