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Literature summary extracted from

  • Roy, S.; Fortin, M.; Gagnon, J.; Ghinet, M.G.; LeHoux, J.; Dupuis, G.; Brzezinski, R.
    Quantitative fluorometric analysis of the protective effect of chitosan on thermal unfolding of catalytically active native and genetically-engineered chitosanases (2007), Biochim. Biophys. Acta, 1774, 975-984.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.132 analysis fluorimetric determination of transition temperature is a reliable method for rapid assessment of the thermal behaviour of chitosanases and applicable to structure-function studies Streptomyces coelicolor
3.2.1.132 analysis fluorimetric determination of transition temperature is a reliable method for rapid assessment of the thermal behaviour of chitosanases and applicable to structure-function studies Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.132 additional information consensus enzyme mutant designed by multiple amino acid substitutions. Increase in transition temperature Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Streptomyces coelicolor
-
 strain strain A3
-
3.2.1.132 Streptomyces sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O substrate with average molecular weight of 220 kDA, 85% deacetylated Streptomyces coelicolor chitosan oligosaccharides
-
 ?
3.2.1.132 chitosan + H2O substrate with average molecular weight of 220 kDA, 85% deacetylated Streptomyces sp. chitosan oligosaccharides
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 43.4
-
 transition temperature in absence of chitosan Streptomyces coelicolor
3.2.1.132 54
-
 wild-type, transition temperature in absence of chitosan Streptomyces sp.
3.2.1.132 61
-
 both wild-type and mutants A104L, K164R are active over 90 min Streptomyces sp.
3.2.1.132 68.6
-
 transition temperature in presence of chitosan at 6 g/l Streptomyces coelicolor
3.2.1.132 69.5
-
 wild-type, transition temperature in presence of chitosan at 6 g/l Streptomyces sp.
3.2.1.132 81.1
-
 consensus mutant, transition temperature in presence of chitosan at 6 g/l Streptomyces sp.
3.2.1.132 83.3
-
 mutant A104L, transition temperature in presence of chitosan at 6 g/l Streptomyces sp.
3.2.1.132 85.1
-
 mutant K164R, transition temperature in presence of chitosan at 6 g/l Streptomyces sp.