Literature summary extracted from
Frase, H.; Lee, I.
Peptidyl boronates inhibit Salmonella enterica serovar Typhimurium Lon protease by a competitive ATP-dependent mechanism (2007), Biochemistry, 46, 6647-6657.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.4.21.53 |
medicine |
lon is a target in the development of antibiotics |
Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.21.53 |
recombinant wild-type and mutant lon overexpressed in Escherichia coli BL21 (DE3), using plasmids pHF020 and pHF031, respectively |
Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.21.53 |
S680A |
displays both intrinsic and peptide-stimulated ATP hydrolysis activity comparable to that of the wild-type enzyme but is unable to catalyze peptide bond hydrolysis. Active site serine is required for interaction of inhibitor with lon |
Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.21.53 |
dansyl-YRGIT-Abu |
not time-dependent inhibitor of peptide hydrolysis activity of lon |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
3.4.21.53 |
dansyl-YRGIT-Abu-B(OH)2 |
potent time-dependent inhibitor of peptide hydrolysis activity of lon, inhibition requires the binding but not hydrolysis of ATP, is an alternative peptidyl boronate inhibitor |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
3.4.21.53 |
MG262 |
potent time-dependent inhibitor of peptide hydrolysis activity of lon, inhibition requires the binding but not hydrolysis of ATP |
Salmonella enterica subsp. enterica serovar Typhimurium |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.21.53 |
Salmonella enterica subsp. enterica serovar Typhimurium |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.21.53 |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.21.53 |
YRGIT-Abu-SGRQK(Bz) + H2O |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.21.53 |
lon protease |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |