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Literature summary extracted from

  • Constantine, C.Z.; Starks, C.M.; Mill, C.P.; Ransome, A.E.; Karpowicz, S.J.; Francois, J.A.; Goodman, R.A.; Kappock, T.J.
    Biochemical and structural studies of N5-carboxyaminoimidazole ribonucleotide mutase from the acidophilic bacterium Acetobacter aceti (2006), Biochemistry, 45, 8193-8208.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.18 enzyme is active in Escherichia coli Acetobacter aceti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.18 mutant H59N soaked in 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, protonation of 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate C4 may occur in absence of H59 Acetobacter aceti

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.18 H59A inactive Acetobacter aceti
5.4.99.18 H59D 0.16% of wild-type activity Acetobacter aceti
5.4.99.18 H59F inactive Acetobacter aceti
5.4.99.18 H59N inactive, crystallization data Acetobacter aceti
5.4.99.18 H59Q almost inactive Acetobacter aceti
5.4.99.18 H59S inactive Acetobacter aceti
5.4.99.18 H89D 4.4% of wild-type activity Acetobacter aceti
5.4.99.18 H89F 14% of wild-type activity Acetobacter aceti
5.4.99.18 H89G 91% of wild-type activity Acetobacter aceti
5.4.99.18 H89N 2.9% of wild-type activity Acetobacter aceti
5.4.99.18 H89V 17% of wild-type activity Acetobacter aceti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.18 0.0007
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H89G, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.0014
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H89N, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.006
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H89D, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.008
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate wild-type, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.008
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H89V, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.009
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H89F, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.03
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H59Q, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.2
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H59D, 37°C, pH 8.0 Acetobacter aceti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.18 18864
-
 8 * 18900, calculated, 8 * 21000, SDS-PAGE, 8 * 18864, ESI-MS Acetobacter aceti
5.4.99.18 18900
-
 8 * 18900, calculated, 8 * 21000, SDS-PAGE, 8 * 18864, ESI-MS Acetobacter aceti
5.4.99.18 21000
-
 8 * 18900, calculated, 8 * 21000, SDS-PAGE, 8 * 18864, ESI-MS Acetobacter aceti
5.4.99.18 197000
-
 gel filtration Acetobacter aceti

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.18 Acetobacter aceti Q2QJL2
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.99.18 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate H89 is not essential and does not function as part of a proton relay system. For synthesis of 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the carboxylate moiety is positioned in a small pocket proposed to facilitate nucleotide decarboxylation. In the reverse direction, enzyme favors protonation of 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate C4 Acetobacter aceti
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.18 36
-
 pH 8.0, 37°C, direction of decarboxylation of 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate Acetobacter aceti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.18 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
-
 Acetobacter aceti 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
-
 r
5.4.99.18 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
-
 Acetobacter aceti 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
-
 r

Subunits

EC Number Subunits Comment Organism
5.4.99.18 octamer 8 * 18900, calculated, 8 * 21000, SDS-PAGE, 8 * 18864, ESI-MS Acetobacter aceti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.18 85
-
 melting temperature Acetobacter aceti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.18 0.0006
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H59Q, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.063
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H89N, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.4
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H89D, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.49
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H59D, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 0.97
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H89G, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 1.97
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H89F, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 2.1
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate mutant H89V, 37°C, pH 8.0 Acetobacter aceti
5.4.99.18 12.2
-
 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate wild-type, 37°C, pH 8.0 Acetobacter aceti

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.4.99.18 7
-
 maximum of enzyme stability near pH 7.0 Acetobacter aceti