Literature summary extracted from
Li, L.; Marsh, E.N.
Deuterium isotope effects in the unusual addition of toluene to fumarate catalyzed by benzylsuccinate synthase (2006), Biochemistry, 45, 13932-13938.
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.1.99.11 |
98000 |
- |
98000, SDS-PAGE of the alpha subunit |
Thauera aromatica |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.1.99.11 |
toluene + fumarate |
Thauera aromatica |
first step in the anaerobic degradation pathway of toluene |
benzylsuccinate |
- |
ir |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.99.11 |
Thauera aromatica |
- |
grown with toluene as carbon source |
- |
Oxidation Stability
EC Number |
Oxidation Stability |
Organism |
---|
4.1.99.11 |
extremely oxygen sensitive |
Thauera aromatica |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.1.99.11 |
loss of activity during purification due to high oxygen sensitivity |
Thauera aromatica |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
4.1.99.11 |
benzylsuccinate = toluene + fumarate |
mechanism |
Thauera aromatica |
|
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.1.99.11 |
0.0024 |
- |
cell extract, 4°C |
Thauera aromatica |
4.1.99.11 |
0.0074 |
- |
cell extract, 30°C |
Thauera aromatica |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.99.11 |
toluene + fumarate |
first step in the anaerobic degradation pathway of toluene |
Thauera aromatica |
benzylsuccinate |
- |
ir |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.1.99.11 |
? |
98000, SDS-PAGE of the alpha subunit |
Thauera aromatica |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
4.1.99.11 |
0.11 |
- |
Toluene |
cell extract, 4°C |
Thauera aromatica |
|
4.1.99.11 |
0.35 |
- |
Toluene |
cell extract, 30°C |
Thauera aromatica |
|