Literature summary extracted from

Huang, H.; Luo, H.; Yang, P.; Meng, K.; Wang, Y.; Yuan, T.; Bai, Y.; Yao, B.
A novel phytase with preferable characteristics from Yersinia intermedia (2006), Biochem. Biophys. Res. Commun., 350, 884-889.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.1.3.26	additional information	not influenced by 1 mM EDTA	Yersinia intermedia

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.3.26	expressed in Pichia pastoris	Yersinia intermedia

General Stability

EC Number	General Stability	Organism
3.1.3.26	strong resistance to proteases, with 90% of activity remaining after treatment with pepsin and trypsin at 37°C for 60 min	Yersinia intermedia

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.1.3.26	Fe2+	47% inhibition at 1 mM	Yersinia intermedia
3.1.3.26	additional information	not inhibited by 1 mM EDTA	Yersinia intermedia
3.1.3.26	SDS	almost completely inhibited at 1 mM	Yersinia intermedia
3.1.3.26	Zn2+	44% inhibition at 1 mM	Yersinia intermedia

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.1.3.26	additional information	not influenced by K+, Co2+, Cr3+, Cu2+, Mg2+, Mn2+, Ag+, and Ca2+	Yersinia intermedia

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.3.26	45000	-	SDS-PAGE	Yersinia intermedia

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.3.26	Yersinia intermedia	Q000T0	-	-
3.1.3.26	Yersinia intermedia H-27	Q000T0	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
3.1.3.26	glycoprotein	-	Yersinia intermedia

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.3.26	HiTrap Q Sepharose XL FPLC column chromatography and Sephacryl S-200 HR gel filtration	Yersinia intermedia

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.3.26	3960	-	with 4 mM sodium phytate in 0.25 M sodium acetate buffer, pH 4.5, at 37°C	Yersinia intermedia

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.3.26	myo-inositol hexakisphosphate + H2O	The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate.	Yersinia intermedia	myo-inositol pentakisphosphate + phosphate	-	?
3.1.3.26	myo-inositol hexakisphosphate + H2O	The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate.	Yersinia intermedia H-27	myo-inositol pentakisphosphate + phosphate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.3.26	AppA	-	Yersinia intermedia

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.1.3.26	55	-	-	Yersinia intermedia

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.1.3.26	60	80	enzyme activity is above 60% after treatment at 80°C for 10 min, and 54% of the activity is retained at 80°C for 15 min	Yersinia intermedia

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.1.3.26	4.5	-	-	Yersinia intermedia

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
3.1.3.26	2	6	above 70% of maximum activity over pH 2.5-5.5	Yersinia intermedia

pH Stability

EC Number	pH Stability	pH Stability Maximum	Comment	Organism
3.1.3.26	1	3	retains 83% of activity at pH range 1-3	Yersinia intermedia
3.1.3.26	3.5	10	retains 93% of activity at pH range 3.5-10	Yersinia intermedia

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Release 2023.1 (January 2023)