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Literature summary extracted from

  • Simkhada, J.R.; Cho, S.S.; Lee, H.J.; Yoo, J.C.
    Purification and biochemical properties of phospholipase D (PLD57) produced by Streptomyces sp. CS-57 (2007), Arch. Pharm. Res., 30, 1302-1308.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 Triton X-100 0.375%, 75% increase in activity Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 deoxycholic acid 0.45%, 26% residual activity Streptomyces sp.
3.1.4.4 FeSO4 2 mM, 78% of initial activity Streptomyces sp.
3.1.4.4 N-laurylsarcosine 0.45%, 0% residual activity Streptomyces sp.
3.1.4.4 polyoxyethylene-4-lauryl ether 0.45%, 21% residual activity Streptomyces sp.
3.1.4.4 sodium dodecylsulfate 0.45%, 3% residual activity Streptomyces sp.
3.1.4.4 Tween 20 0.45%, 3% residual activity Streptomyces sp.
3.1.4.4 Tween 80 0.45%, 5% residual activity Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.4 extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 CoSO4 2 mM, 106% of initial activity Streptomyces sp.
3.1.4.4 EDTA 2 mM, 139% of initial activity Streptomyces sp.
3.1.4.4 MnSO4 2 mM, 116% of initial activity Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 55000
-
 x * 55000, SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Streptomyces sp.
-
-
-
3.1.4.4 Streptomyces sp. CS-57
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 30
-
 pH 8.0, 55°C Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 additional information in presence of glycerol, the rate of hydrolysis of phosphatiylcholine and the rate of phosphytidylglycerol formation are almost identical Streptomyces sp. ?
-
 ?
3.1.4.4 additional information in presence of glycerol, the rate of hydrolysis of phosphatiylcholine and the rate of phosphytidylglycerol formation are almost identical Streptomyces sp. CS-57 ?
-
 ?
3.1.4.4 phosphatidylcholine + glycerol
-
 Streptomyces sp. phosphatidylglycerol + choline
-
 ?
3.1.4.4 phosphatidylcholine + glycerol
-
 Streptomyces sp. CS-57 phosphatidylglycerol + choline
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Streptomyces sp. 1,2-diacylglycerophosphate + choline
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Streptomyces sp. CS-57 1,2-diacylglycerophosphate + choline
-
 ?
3.1.4.4 phosphatidylcholine + inositol
-
 Streptomyces sp. phosphatidylinositol + choline
-
 ?
3.1.4.4 phosphatidylcholine + inositol
-
 Streptomyces sp. CS-57 phosphatidylinositol + choline
-
 ?
3.1.4.4 phosphatidylcholine + L-serine
-
 Streptomyces sp. phosphatidylserine + choline
-
 ?
3.1.4.4 phosphatidylcholine + L-serine
-
 Streptomyces sp. CS-57 phosphatidylserine + choline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.4 ? x * 55000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 35
-
 transphosphatidylation activity Streptomyces sp.
3.1.4.4 45
-
 hydrolytic activity Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.4 45
-
 2 h, no loss of activity Streptomyces sp.
3.1.4.4 55
-
 20 min, complete loss of activity Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 7.5
-
 both hydrolytic and transphosphatidylation activity Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.4 6 8
-
 Streptomyces sp.