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Literature summary extracted from

  • Landmann, C.; Fink, B.; Festner, M.; Dregus, M.; Engel, K.H.; Schwab, W.
    Cloning and functional characterization of three terpene synthases from lavender (Lavandula angustifolia) (2007), Arch. Biochem. Biophys., 465, 417-429.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.20 expression in Escherichia coli Lavandula angustifolia
4.2.3.20 into the pGEM-T vector for sequencing and into the pGEX-T-1 vector for expression in Escherichia coli cells Lavandula angustifolia
4.2.3.81
-
 Lavandula angustifolia
4.2.3.113 expression in Escherichia coli Lavandula angustifolia
4.2.3.121 expression in Escherichia coli Lavandula angustifolia

General Stability

EC Number General Stability Organism
4.2.3.81 bovine serum albumin stabilizes Lavandula angustifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.20 0.0474
-
 geranyl diphosphate pH 7.0, 30°C Lavandula angustifolia
4.2.3.20 0.0474
-
 geranyl diphosphate LaLIMS_RR, truncated protein Lavandula angustifolia
4.2.3.81 0.0047
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 23°C Lavandula angustifolia
4.2.3.113 0.0474
-
 geranyl diphosphate pH 7.0, 30°C Lavandula angustifolia
4.2.3.121 0.0474
-
 geranyl diphosphate pH 7.5, 23°C Lavandula angustifolia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.20 Mg2+
-
 Lavandula angustifolia
4.2.3.20 Mg2+ or Mn2+, reuired. Optimum concentraion 50 mM Lavandula angustifolia
4.2.3.20 Mn2+ the proportions of the products change significantly when Mn2+ is supplied instead of Mg2+ Lavandula angustifolia
4.2.3.20 Mn2+ or Mg2+, required. Optimum concentration 0.2 mM, 68% of the efficieny with Mg2+. Product pattern changes markedly to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+ Lavandula angustifolia
4.2.3.81 Mg2+ product formation is highest in the presence of the cofactor Mg2+ with product concentration peaking at 50 mM Lavandula angustifolia
4.2.3.81 Mn2+ the assays with Mn2+ shows highest activity at 0.05 mM with 49% of the maximum product level Lavandula angustifolia
4.2.3.113 Mg2+ or Mn2+, reuired. Optimum concentraion 50 mM Lavandula angustifolia
4.2.3.113 Mn2+ or Mg2+, required. Optimum concentration 0.2 mM, 68% of the efficieny with Mg2+. Product pattern changes markedly to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+ Lavandula angustifolia
4.2.3.116 Mg2+ or Mn2+, required, Mg2+ is preferred over Mn2+. Optimum concentration 50 mM Lavandula angustifolia
4.2.3.116 Mn2+ or Mg2+, required. Optimum concentration 0.2 mM, 68% of the efficiency with Mg2+ Lavandula angustifolia
4.2.3.121 Mg2+ or Mn2+, required, optimum concentration 50 mM Lavandula angustifolia
4.2.3.121 Mn2+ or Mg2+, required, optimum concentration 0.2 mM. 68% of the efficiency with Mg2+, with change in product pattern to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, for Mn2+ Lavandula angustifolia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.20 70300
-
 x * 70300, SDS-PAGE Lavandula angustifolia
4.2.3.113 70300
-
 x * 70300, SDS-PAGE Lavandula angustifolia
4.2.3.121 70300
-
 x * 70300, calculated Lavandula angustifolia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.20 geranyl diphosphate Lavandula angustifolia
-
 (+)-(4R)-limonene + diphosphate
-
 ?
4.2.3.81 (2E,6E)-farnesyl diphosphate Lavandula angustifolia
-
 (-)-exo-alpha-bergamotene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.20 Lavandula angustifolia Q2XSC6
-
-
4.2.3.81 Lavandula angustifolia Q2XSC4
-
-
4.2.3.113 Lavandula angustifolia Q2XSC6
-
-
4.2.3.116 Lavandula angustifolia Q2XSC6
-
-
4.2.3.121 Lavandula angustifolia Q2XSC6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.20 using GST bind resin Lavandula angustifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.20 flower
-
 Lavandula angustifolia
-
4.2.3.20 leaf
-
 Lavandula angustifolia
-
4.2.3.113 flower
-
 Lavandula angustifolia
-
4.2.3.113 leaf
-
 Lavandula angustifolia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.20 geranyl diphosphate
-
 Lavandula angustifolia (+)-(4R)-limonene + diphosphate
-
 ?
4.2.3.20 geranyl diphosphate
-
 Lavandula angustifolia (1R,5R)-(+)-alpha-pinene + diphosphate
-
 ?
4.2.3.20 geranyl diphosphate
-
 Lavandula angustifolia (1R,4S)-(+)-camphene + diphosphate
-
 ?
4.2.3.20 geranyl diphosphate
-
 Lavandula angustifolia alpha-phellandrene + diphosphate
-
 ?
4.2.3.20 geranyl diphosphate
-
 Lavandula angustifolia beta-myrcene + diphosphate
-
 ?
4.2.3.20 geranyl diphosphate
-
 Lavandula angustifolia terpinolene + diphosphate
-
 ?
4.2.3.20 geranyl diphosphate
-
 Lavandula angustifolia (R)-limonene + diphosphate products are 39% (R)-limonene, 22% terpinolene, 16% (1R,5S)-camphene, 14% (1R,5R)-(+)-alpha-pinene, 8% betamyrcene and traces of alpha-phellandrene. Product pattern changes markedly to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+ ?
4.2.3.20 additional information no substrates: farnesyl diphosphate, geranylgeranyl diphosphate Lavandula angustifolia ?
-
 ?
4.2.3.81 (2E,6E)-farnesyl diphosphate
-
 Lavandula angustifolia (-)-exo-alpha-bergamotene + diphosphate
-
 ?
4.2.3.113 geranyl diphosphate
-
 Lavandula angustifolia terpinolene + diphosphate products are 39% (R)-(+)-limonene, 22% terpinolene, 16% (1R,5S)-(+)-camphene, 14% (1R,5R)-(+)-alpha-pinene, 8% betamyrcene and traces of alpha-phellandrene. Product pattern changes markedly to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% a-phellandrene, when Mn2+ is supplied instead of Mg2+ ?
4.2.3.113 additional information no substrates: farnesyl diphosphate, geranylgeranyl diphosphate Lavandula angustifolia ?
-
 ?
4.2.3.116 geranyl diphosphate
-
 Lavandula angustifolia (+)-camphene + diphosphate products are 39% (+)-limonene, 22% terpinolene, 16% (+)-camphene, 14% (+)-alpha-pinene, 8% beta-myrcene, 1% alpha-phellandrene. Product pattern changes markedly to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+ ?
4.2.3.116 additional information no substrate: farnesyl diphosphate, geranylgeranyl diphosphate Lavandula angustifolia ?
-
 ?
4.2.3.121 geranyl diphosphate
-
 Lavandula angustifolia (+)-alpha-pinene + diphosphate products are 39% (R)-(+)-limonene, 22% terpinolene, 16% (1R,5S)-(+)-camphene, 14% (1R,5R)-(+)-alpha-pinene, 8% beta-myrcene and traces of alpha-phellandrene. Preferential formation of the (+)-enantiomers with 94% enantiomeric purity for (1R,5R)-(+)-alpha-pinene. Product pattern changes to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+ ?

Subunits

EC Number Subunits Comment Organism
4.2.3.20 ? x * 70300, SDS-PAGE Lavandula angustifolia
4.2.3.113 ? x * 70300, SDS-PAGE Lavandula angustifolia
4.2.3.121 ? x * 70300, calculated Lavandula angustifolia

Synonyms

EC Number Synonyms Comment Organism
4.2.3.20 (R)-limonene synthase
-
 Lavandula angustifolia
4.2.3.20 LaLIMS
-
 Lavandula angustifolia
4.2.3.20 limonene synthase
-
 Lavandula angustifolia
4.2.3.20 LIMS
-
 Lavandula angustifolia
4.2.3.81 LaBERS
-
 Lavandula angustifolia
4.2.3.113 limonene synthase
-
 Lavandula angustifolia
4.2.3.113 LIMS
-
 Lavandula angustifolia
4.2.3.121 limonene synthase
-
 Lavandula angustifolia
4.2.3.121 LIMS
-
 Lavandula angustifolia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.20 23
-
 standard activity assay Lavandula angustifolia
4.2.3.20 30
-
-
 Lavandula angustifolia
4.2.3.20 30
-
 optimized assay, LaLIMS_RR, truncated protein Lavandula angustifolia
4.2.3.81 23
-
 assay at Lavandula angustifolia
4.2.3.81 30
-
-
 Lavandula angustifolia
4.2.3.113 30
-
-
 Lavandula angustifolia
4.2.3.116 30
-
-
 Lavandula angustifolia
4.2.3.121 30
-
 optimum for total enzymic activity Lavandula angustifolia
4.2.3.121 33
-
 optimum for maximum formation of alpha-pinene Lavandula angustifolia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.20 0.012
-
 geranyl diphosphate LaLIMS_RR, truncated protein Lavandula angustifolia
4.2.3.81 0.033
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 23°C Lavandula angustifolia
4.2.3.121 0.012
-
 geranyl diphosphate pH 7.5, 23°C Lavandula angustifolia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.20 7
-
-
 Lavandula angustifolia
4.2.3.20 7
-
 optimized assay, LaLIMS_RR, truncated protein Lavandula angustifolia
4.2.3.20 7.5
-
 standard activity assay Lavandula angustifolia
4.2.3.81 7.5
-
 assay at Lavandula angustifolia
4.2.3.81 8
-
-
 Lavandula angustifolia
4.2.3.113 7
-
-
 Lavandula angustifolia
4.2.3.116 7
-
-
 Lavandula angustifolia
4.2.3.121 6
-
 optimum for maximum formation of alpha-pinene Lavandula angustifolia
4.2.3.121 7
-
 optimum for total enzymic activity Lavandula angustifolia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.20 0.255
-
 geranyl diphosphate pH 7.0, 30°C Lavandula angustifolia
4.2.3.81 0.7
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 23°C Lavandula angustifolia
4.2.3.113 0.255
-
 geranyl diphosphate pH 7.0, 30°C Lavandula angustifolia
4.2.3.121 0.253
-
 geranyl diphosphate pH 7.5, 23°C Lavandula angustifolia