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Literature summary extracted from

  • Luo, Y.; Zheng, Y.; Jiang, Z.; Ma, Y.; Wei, D.
    A novel psychrophilic lipase from Pseudomonas fluorescens with unique property in chiral resolution and biodiesel production via transesterification (2006), Appl. Microbiol. Biotechnol., 73, 349-355.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.3 additional information LipB68 acts as biocatalysts in biodiesel production and produces biodiesel with a yield of 92% after 12 h, at the lowest temperature of 20°C, catalyzing the transesterification reaction of biodiesel production, thus LipB68 represents a highly competitive energy-saving biocatalyst Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 gene lipB68, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21(DE3) inclusion bodies Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas fluorescens Q670W0 isolated from soil samples of China, gene lipB68
-
3.1.1.3 Pseudomonas fluorescens B68 Q670W0 isolated from soil samples of China, gene lipB68
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant LipB68 protein from Escherichia coli strain BL21(DE3) after solubilization from inclusion bodies, to 95% purity Pseudomonas fluorescens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.3 recombinant LipB68 from Escherichia coli inclusion bodies by 8 M urea and subsequent dialysis, 25 mM Tris-HCl, pH 8.0 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl caprate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + caprate
-
 ?
3.1.1.3 4-nitrophenyl caprate + H2O
-
 Pseudomonas fluorescens B68 4-nitrophenol + caprate
-
 ?
3.1.1.3 additional information LipB68 performs the enantioselective acylation of racemic alcohols, e.g. toluene, and effectively catalyzes the transesterification of both alpha-phenylethanol and alpha-phenylpropanol at 20°C with high enantioselectivity, overview Pseudomonas fluorescens ?
-
 ?
3.1.1.3 additional information LipB68 performs the enantioselective acylation of racemic alcohols, e.g. toluene, and effectively catalyzes the transesterification of both alpha-phenylethanol and alpha-phenylpropanol at 20°C with high enantioselectivity, overview Pseudomonas fluorescens B68 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 LipB68
-
 Pseudomonas fluorescens
3.1.1.3 More the enzyme is a member of lipase subfamily I.3 Pseudomonas fluorescens
3.1.1.3 psychrophilic lipase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 20
-
-
 Pseudomonas fluorescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 5 50 temperature profile, overview Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
 assay at Pseudomonas fluorescens