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Literature summary extracted from
- Extraction and partial characterization of polyphenol oxidase from banana (Musa acuminata Grande naine) roots (2006), Plant Physiol. Biochem., 44, 308-314.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.1 | 4-hydroxybenzoic acid | - |
Musa acuminata |  |
| 1.10.3.1 | Citric acid | slight inhibition | Musa acuminata | |
| 1.10.3.1 | dithiothreitol | complete inhibition at 0.150 mM of the root enzyme | Musa acuminata | |
| 1.10.3.1 | EDTA | inhibits the root enzyme, while the pulp enzyme is only poorly inhiibited | Musa acuminata | |
| 1.10.3.1 | L-ascorbic acid | complete inhibition at 0.90 mM of the root enzyme, and at 1 mM of the pulp enzyme | Musa acuminata | |
| 1.10.3.1 | L-cysteine | complete inhibition at 0.973 mM of the root enzyme, and at 1 mM of the pulp enzyme | Musa acuminata | |
| 1.10.3.1 | Sodium metabisulfite | complete inhibition at 0.109 mM of the root enzyme, and at 1 mM of the pulp enzyme | Musa acuminata | |
| 1.14.18.1 | dithiothreitol | strong inhibition | Musa acuminata | |
| 1.14.18.1 | Sodium metabisulfite | strong inhibition | Musa acuminata | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.1 | additional information | - |
additional information | Michaelis-Menten kinetics | Musa acuminata | |
| 1.10.3.1 | 0.6 | - |
dopamine | pH 6.5, 25°C | Musa acuminata | |
| 1.10.3.1 | 1.2 | - |
D-catechin | pH 6.5, 25°C | Musa acuminata | |
| 1.10.3.1 | 6.2 | - |
chlorogenic acid | pH 6.5, 25°C | Musa acuminata | |
| 1.10.3.1 | 8.3 | - |
4-methylcatechol | pH 6.5, 25°C | Musa acuminata | |
| 1.10.3.1 | 9.5 | - |
caffeic acid | pH 6.5, 25°C | Musa acuminata | |
| 1.10.3.1 | 56.3 | - |
L-Dopa | pH 6.5, 25°C | Musa acuminata | |
| 1.10.3.1 | 69 | - |
catechol | pH 6.5, 25°C | Musa acuminata | |
| 1.14.18.1 | additional information | - |
additional information | no Michaelis-Menten kinetics | Musa acuminata |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.10.3.1 | D-catechin + O2 | Musa acuminata | - |
? | - |
? | |
| 1.10.3.1 | dopamine + O2 | Musa acuminata | - |
? | - |
? | |
| 1.10.3.1 | additional information | Musa acuminata | the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plants defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis | ? | - |
? | |
| 1.14.18.1 | additional information | Musa acuminata | the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plants defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.10.3.1 | Musa acuminata | - |
- |
- |
| 1.14.18.1 | Musa acuminata | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.10.3.1 | optimization of enzyme extraction from roots, best using 0.2 M phosphate buffer at pH 7.0 with 5% insoluble polyvinylpyrrolidone and 0.25% Triton X-100, overview | Musa acuminata |
| 1.14.18.1 | optimization of enzyme extraction from roots, best using 0.2 M phosphate buffer at pH 7.0 with 5% insoluble polyvinylpyrrolidone and 0.25% Triton X-100, overview | Musa acuminata |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.10.3.1 | fruit | pulp and peel | Musa acuminata | - |
| 1.10.3.1 | leaf | - |
Musa acuminata | - |
| 1.10.3.1 | additional information | tissue distribution, overview | Musa acuminata | - |
| 1.10.3.1 | root | aerenchyma in the cortex | Musa acuminata | - |
| 1.10.3.1 | stem | - |
Musa acuminata | - |
| 1.14.18.1 | fruit | pulp and peel | Musa acuminata | - |
| 1.14.18.1 | leaf | - |
Musa acuminata | - |
| 1.14.18.1 | additional information | tissue distribution, overview | Musa acuminata | - |
| 1.14.18.1 | pulp | - |
Musa acuminata | - |
| 1.14.18.1 | root | - |
Musa acuminata | - |
| 1.14.18.1 | stem | - |
Musa acuminata | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.1 | additional information | - |
- |
Musa acuminata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.10.3.1 | 4-methylcatechol + O2 | - |
Musa acuminata | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
| 1.10.3.1 | caffeic acid + O2 | - |
Musa acuminata | ? | - |
? | |
| 1.10.3.1 | catechol + 1/2 O2 | low activity | Musa acuminata | 1,2-benzoquinone + H2O | - |
? | |
| 1.10.3.1 | chlorogenic acid + O2 | - |
Musa acuminata | ? | - |
? | |
| 1.10.3.1 | D-catechin + O2 | - |
Musa acuminata | ? | - |
? | |
| 1.10.3.1 | D-catechin + O2 | best substrate | Musa acuminata | ? | - |
? | |
| 1.10.3.1 | dopamine + O2 | - |
Musa acuminata | ? | - |
? | |
| 1.10.3.1 | L-dopa + 1/2 O2 | i.e. L-3,4-dihydroxyphenylalanine, low activity | Musa acuminata | L-dopaquinone + H2O | - |
? | |
| 1.10.3.1 | additional information | the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plants defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis | Musa acuminata | ? | - |
? | |
| 1.10.3.1 | additional information | the enzyme catalyzes the hydroxylation of monophenols to o-diphenols, monophenolase activity EC 1.14.18.1, and the oxidation of the o-diphenols to o-quinones, diphenolase activity EC 1.10.3.1, cross-reaction analysis, overview | Musa acuminata | ? | - |
? | |
| 1.14.18.1 | 4-cresol + O2 | root and pulp enzyme | Musa acuminata | ? | - |
? | |
| 1.14.18.1 | additional information | the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plants defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis | Musa acuminata | ? | - |
? | |
| 1.14.18.1 | additional information | no activity with L-tyrosine by the root and the pulp enzyme, the enzyme catalyzes the hydroxylation of monophenols to o-diphenols, monophenolase activity EC 1.14.18.1, and the oxidation of the o-diphenols to o-quinones, diphenolase activity EC 1.10.3.1, cross-reaction analysis, overview | Musa acuminata | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.10.3.1 | catecholase | - |
Musa acuminata |
| 1.10.3.1 | More | cf. 1.14.18.1 | Musa acuminata |
| 1.10.3.1 | o-diphenoloxidase | - |
Musa acuminata |
| 1.10.3.1 | polyphenol oxidase | - |
Musa acuminata |
| 1.10.3.1 | PPO | - |
Musa acuminata |
| 1.14.18.1 | cresolase | - |
Musa acuminata |
| 1.14.18.1 | More | cf. EC 1.10.3.1 | Musa acuminata |
| 1.14.18.1 | polyphenol oxidase | - |
Musa acuminata |
| 1.14.18.1 | PPO | - |
Musa acuminata |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.1 | 25 | - |
assay at | Musa acuminata |
| 1.14.18.1 | 25 | - |
assay at | Musa acuminata |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.1 | 6.5 | - |
assay at | Musa acuminata |
| 1.14.18.1 | 6.5 | - |
assay at | Musa acuminata |
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