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Literature summary extracted from

  • Scarponi, L.; Quagliarini, E.; Del Buono, D.
    Induction of wheat and maize glutathione S-transferase by some herbicide safeners and their effect on enzyme activity against butachlor and terbuthylazine (2006), Pest Manag. Sci., 62, 927-932.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.18 benoxacor herbicide safener, activates the enzyme in shoots Triticum aestivum
2.5.1.18 benoxacor herbicide safener, activates the enzyme in shoots Zea mays
2.5.1.18 cloquintocet-mexyl herbicide safener, activates the enzyme in shoots Triticum aestivum
2.5.1.18 cloquintocet-mexyl herbicide safener, activates the enzyme in shoots Zea mays
2.5.1.18 fenchlorazole-ethyl herbicide safener, activates the enzyme in shoots Triticum aestivum
2.5.1.18 fenchlorazole-ethyl herbicide safener, activates the enzyme in shoots Zea mays
2.5.1.18 fenclorim herbicide safener, activates the enzyme in shoots Triticum aestivum
2.5.1.18 fenclorim herbicide safener, activates the enzyme in shoots Zea mays
2.5.1.18 fluxofenim herbicide safener, activates the enzyme in shoots Triticum aestivum
2.5.1.18 fluxofenim herbicide safener, activates the enzyme in shoots Zea mays
2.5.1.18 additional information no enzyme activation by oxabetrinil, a herbicide safener Zea mays
2.5.1.18 oxabetrinil herbicide safener, activates the enzyme in shoots Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.557
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of cloquintocet-mexyl Triticum aestivum
2.5.1.18 0.572
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fluxofenim Triticum aestivum
2.5.1.18 0.577
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fenclorim Triticum aestivum
2.5.1.18 0.629
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fenchlorazole-ethyl Triticum aestivum
2.5.1.18 0.655
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of benoxacor Triticum aestivum
2.5.1.18 0.749
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C Triticum aestivum
2.5.1.18 0.753
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of oxabetrinil Triticum aestivum
2.5.1.18 1.07
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fluxofenim Zea mays
2.5.1.18 1.081
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of cloquintocet-mexyl Zea mays
2.5.1.18 1.342
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of benoxacor Zea mays
2.5.1.18 1.372
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fenchlorazole-ethyl Zea mays
2.5.1.18 1.442
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of fenclorim Zea mays
2.5.1.18 1.626
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C, in presence of oxabetrinil Zea mays
2.5.1.18 1.79
-
 1-chloro-2,4-dinitrobenzene pH 6.5, 35°C Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Triticum aestivum
-
 cv. Palesio
-
2.5.1.18 Zea mays
-
 cv. Belgrano
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 native enzyme partially from shoots by ammonium sulfate fractionation and desalting Triticum aestivum
2.5.1.18 native enzyme partially from shoots by ammonium sulfate fractionation and desalting Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 seedling
-
 Triticum aestivum
-
2.5.1.18 seedling
-
 Zea mays
-
2.5.1.18 shoot
-
 Triticum aestivum
-
2.5.1.18 shoot
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.000411
-
 substrate butachlor Triticum aestivum
2.5.1.18 0.00087
-
 substrate terbuthylazine Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Triticum aestivum chloride + 2,4-dinitrophenyl-glutathione
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Zea mays chloride + 2,4-dinitrophenyl-glutathione
-
 ?
2.5.1.18 glutathione + butachlor
-
 Triticum aestivum ?
-
 ?
2.5.1.18 glutathione + butachlor
-
 Zea mays ?
-
 ?
2.5.1.18 glutathione + terbuthylazine
-
 Triticum aestivum ?
-
 ?
2.5.1.18 glutathione + terbuthylazine
-
 Zea mays ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Triticum aestivum
2.5.1.18 glutathione S-transferase
-
 Zea mays
2.5.1.18 GST
-
 Triticum aestivum
2.5.1.18 GST
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 35
-
 assay at Triticum aestivum
2.5.1.18 35
-
 assay at Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 6.5
-
 assay at Triticum aestivum
2.5.1.18 6.5
-
 assay at Zea mays