Any feedback?
Literature summary extracted from
- Allosteric NADP-glutamate dehydrogenase from aspergilli: purification, characterization and implications for metabolic regulation at the carbon-nitrogen interface (2005), Microbiology, 151, 1409-1419.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.4 | 3,5-pyrazoledicarboxylate | activates at lower 2-oxoglutarate concentration | Aspergillus niger |  |
| 1.4.1.4 | 3,5-pyridinedicarboxylate | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
| 1.4.1.4 | DL-2-hydroxyglutarate | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
| 1.4.1.4 | Glutarate | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
| 1.4.1.4 | Itaconate | activates even in the presence of 10 mM 2-oxoglutarate | Aspergillus niger | |
| 1.4.1.4 | oxalylglycine | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.4 | 2,4-pyridinedicarboxylate | - |
Aspergillus niger | |
| 1.4.1.4 | 2-Methyleneglutarate | - |
Aspergillus niger | |
| 1.4.1.4 | 2-oxoglutarate | shows allosteric properties and a sigmoid response (nH=2.5) towards 2-oxoglutarate saturation | Aspergillus niger | |
| 1.4.1.4 | isophthalate | - |
Aspergillus niger | |
| 1.4.1.4 | additional information | not inhibited by D-glutamate, L-glutamine or DL-2-hydroxyglutarate | Aspergillus niger | |
| 1.4.1.4 | NADP+ | inhibits at higher 2-oxoglutarate levels | Aspergillus niger | |
| 1.4.1.4 | NH4+ | - |
Aspergillus niger | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.4 | 0.017 | - |
NADP+ | - |
Aspergillus niger | |
| 1.4.1.4 | 1.05 | - |
NH4+ | - |
Aspergillus niger | |
| 1.4.1.4 | 4.78 | - |
2-oxoglutarate | - |
Aspergillus niger | |
| 1.4.1.4 | 34.7 | - |
L-glutamate | - |
Aspergillus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.4 | 50000 | - |
x * 50000, SDS-PAGE | Aspergillus niger |
| 1.4.1.4 | 350000 | - |
gel filtration | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.4 | Aspergillus awamori | - |
- |
- |
| 1.4.1.4 | Aspergillus awamori NCIM 1225 | - |
- |
- |
| 1.4.1.4 | Aspergillus nidulans | - |
- |
- |
| 1.4.1.4 | Aspergillus nidulans NCIM 1211 | - |
- |
- |
| 1.4.1.4 | Aspergillus niger | - |
- |
- |
| 1.4.1.4 | Aspergillus niger NCIM 565 | - |
- |
- |
| 1.4.1.4 | Aspergillus oryzae | - |
- |
- |
| 1.4.1.4 | Aspergillus oryzae NCIM 553 | - |
- |
- |
| 1.4.1.4 | Aspergillus terreus | - |
- |
- |
| 1.4.1.4 | Aspergillus terreus NCIM 656 | - |
- |
- |
| 1.4.1.4 | Saccharomyces cerevisiae | - |
strain Y05499, strain Y10345 and strain Y11672 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.4 | by Cibacron red LS-B dye-affinity purification with a step KCl gradient elution, 17fold purified | Aspergillus nidulans |
| 1.4.1.4 | by Cibacron red LS-B dye-affinity purification with a step KCl gradient elution, more than 7fold purified to homogeneity | Aspergillus niger |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.4.1.4 | mycelium | - |
Aspergillus nidulans | - |
| 1.4.1.4 | mycelium | - |
Aspergillus niger | - |
| 1.4.1.4 | mycelium | - |
Aspergillus oryzae | - |
| 1.4.1.4 | mycelium | - |
Aspergillus terreus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.4 | 0.37 | - |
crude extract | Aspergillus nidulans |
| 1.4.1.4 | 2.17 | - |
crude extract | Aspergillus niger |
| 1.4.1.4 | 7 | - |
purified enzyme | Aspergillus nidulans |
| 1.4.1.4 | 59.4 | - |
purified enzyme | Aspergillus niger |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.4.1.4 | 4°C, 1 week | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus nidulans | L-glutamate + NADP+ + H2O | - |
r | |
| 1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus niger | L-glutamate + NADP+ + H2O | - |
r | |
| 1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus oryzae | L-glutamate + NADP+ + H2O | - |
r | |
| 1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus terreus | L-glutamate + NADP+ + H2O | - |
r | |
| 1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus awamori | L-glutamate + NADP+ + H2O | - |
r | |
| 1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus oryzae NCIM 553 | L-glutamate + NADP+ + H2O | - |
r | |
| 1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus nidulans NCIM 1211 | L-glutamate + NADP+ + H2O | - |
r | |
| 1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus niger NCIM 565 | L-glutamate + NADP+ + H2O | - |
r | |
| 1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus awamori NCIM 1225 | L-glutamate + NADP+ + H2O | - |
r | |
| 1.4.1.4 | 2-oxoglutarate + NADPH + NH3 | - |
Aspergillus terreus NCIM 656 | L-glutamate + NADP+ + H2O | - |
r | |
| 1.4.1.4 | 2-oxoglutarate + NH4+ + NADPH | - |
Aspergillus niger | L-glutamate + NADP+ | - |
? | |
| 1.4.1.4 | 2-oxoglutarate + NH4+ + NADPH | - |
Aspergillus niger NCIM 565 | L-glutamate + NADP+ | - |
? | |
| 1.4.1.4 | additional information | no reaction with D-glutamate, L-glutamine or DL-2-hydroxyglutarate as possible substrates in place of L-glutamate, methylamine is unable to replace ammonium in the biosynthetic reaction | Aspergillus niger | ? | - |
? | |
| 1.4.1.4 | additional information | no reaction with D-glutamate, L-glutamine or DL-2-hydroxyglutarate as possible substrates in place of L-glutamate, methylamine is unable to replace ammonium in the biosynthetic reaction | Aspergillus niger NCIM 565 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.4.1.4 | hexamer | x * 50000, SDS-PAGE | Aspergillus niger |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.4.1.4 | NADP-GDH | - |
Saccharomyces cerevisiae |
| 1.4.1.4 | NADP-GDH | - |
Aspergillus nidulans |
| 1.4.1.4 | NADP-GDH | - |
Aspergillus niger |
| 1.4.1.4 | NADP-GDH | - |
Aspergillus oryzae |
| 1.4.1.4 | NADP-GDH | - |
Aspergillus terreus |
| 1.4.1.4 | NADP-GDH | - |
Aspergillus awamori |
| 1.4.1.4 | NADP-glutamate dehydrogenase | - |
Saccharomyces cerevisiae |
| 1.4.1.4 | NADP-glutamate dehydrogenase | - |
Aspergillus nidulans |
| 1.4.1.4 | NADP-glutamate dehydrogenase | - |
Aspergillus niger |
| 1.4.1.4 | NADP-glutamate dehydrogenase | - |
Aspergillus oryzae |
| 1.4.1.4 | NADP-glutamate dehydrogenase | - |
Aspergillus terreus |
| 1.4.1.4 | NADP-glutamate dehydrogenase | - |
Aspergillus awamori |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.4 | 8.5 | - |
reductive amination reaction | Aspergillus niger |
| 1.4.1.4 | 9.75 | - |
oxidative deamination reaction | Aspergillus niger |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.4 | NADP+ | - |
Aspergillus nidulans | |
| 1.4.1.4 | NADP+ | - |
Aspergillus oryzae | |
| 1.4.1.4 | NADP+ | - |
Aspergillus terreus | |
| 1.4.1.4 | NADP+ | - |
Aspergillus awamori | |
| 1.4.1.4 | NADP+ | stimulates the enzyme at lower 2-oxoglutarate levels | Aspergillus niger | |
| 1.4.1.4 | NADPH | - |
Aspergillus nidulans | |
| 1.4.1.4 | NADPH | - |
Aspergillus niger | |
| 1.4.1.4 | NADPH | - |
Aspergillus oryzae | |
| 1.4.1.4 | NADPH | - |
Aspergillus terreus | |
| 1.4.1.4 | NADPH | - |
Aspergillus awamori | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.4 | 0.4 | - |
NH4+ | - |
Aspergillus niger | |
| 1.4.1.4 | 6.9 | - |
isophthalate | - |
Aspergillus niger | |
| 1.4.1.4 | 9.2 | - |
2-Methyleneglutarate | - |
Aspergillus niger | |
| 1.4.1.4 | 202 | - |
2,4-pyridinedicarboxylate | - |
Aspergillus niger | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
