Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kai, G.; Jiang, J.; Zhao, D.; Zhao, L.; Zhang, L.; Li, Z.; Guo, B.; Sun, X.; Miao, Z.; Tang, K.
    Isolation and expression profile analysis of a new cDNA encoding 5-alpha-taxadienol-10-beta-hydroxylase from Taxus media (2006), J. Plant Biochem. Biotechnol., 15, 1-5.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.105 additional information elicitors methyl jasmonate, AgNO3, and ammonium ceric sulfate all induce expression of the enzyme Taxus x media

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.105 DNA and amino acid sequence determination and analysis Taxus x media

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.105 57000
-
 x * 57000, about, amino acid sequence calculation Taxus x media

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.105 taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 Taxus x media second P450-dependent step in taxol biosynthesis, regulation mechanism involving elicitors taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.105 Taxus x media
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.105 leaf young, strong expression level Taxus x media
-
1.14.14.105 additional information tissue expression pattern, no expressionin fruits, overview Taxus x media
-
1.14.14.105 stem weak expression level Taxus x media
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.105 taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
 Taxus x media taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.105 taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 second P450-dependent step in taxol biosynthesis, regulation mechanism involving elicitors Taxus x media taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.105 ? x * 57000, about, amino acid sequence calculation Taxus x media

Synonyms

EC Number Synonyms Comment Organism
1.14.14.105 5-alpha-taxadienol-10-beta-hydroxylase
-
 Taxus x media
1.14.14.105 Tm10BH
-
 Taxus x media

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.105 cytochrome P-450
-
 Taxus x media

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.105 Taxus x media amino acid sequence calculation
-
 8.9