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Literature summary extracted from

  • Bol, E.; Bevers, L.E.; Hagedoorn, P.L.; Hagen, W.R.
    Redox chemistry of tungsten and iron-sulfur prosthetic groups in Pyrococcus furiosus formaldehyde ferredoxin oxidoreductase (2006), J. Biol. Inorg. Chem., 11, 999-1006.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.B2 Sulfide incubation of the enzyme (14 mg/ml) in 100 mM Tris/HCl (pH 8.0) buffer with excess sodium sulfide (20 mM) under anaerobic conditions leads to an enhancement of the specific activity by 40% Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.B2 0.014
-
 oxidized ferredoxin pH 8.4, 80°C Pyrococcus furiosus
1.2.7.B2 0.021
-
 formaldehyde pH 8.4, 80°C, with ferredoxin as electron acceptor Pyrococcus furiosus
1.2.7.B2 0.025
-
 formaldehyde pH 8.4, 20°C Pyrococcus furiosus
1.2.7.B2 0.033
-
 formaldehyde pH 8.4, 80°C Pyrococcus furiosus
1.2.7.B2 0.071
-
 formaldehyde pH 8.4, 80°C, with benzyl viologen as electron acceptor Pyrococcus furiosus
1.2.7.B2 0.073
-
 Oxidized benzyl viologen pH 8.4, 80°C Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.B2 Iron contains one [4Fe-4S] cubane per 69000 Da subunit Pyrococcus furiosus
1.2.7.B2 Tungsten contains one tungstodipterin per 69000 Da subunit Pyrococcus furiosus
1.2.7.B2 Tungsten the tungsten in the active enzyme is a two-electron acceptor, W(VI/IV) Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.B2 Pyrococcus furiosus
-
-
-
1.2.7.B2 Pyrococcus furiosus Q8U1K3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.B2 formaldehyde + H2O + 2 oxidized ferredoxin
-
 Pyrococcus furiosus formate + 2 H+ + 2 reduced ferredoxin
-
 ?
1.2.7.B2 formaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus formate + H+ + reduced benzyl viologen
-
 ?
1.2.7.B2 formaldehyde + H2O + oxidized benzyl viologen no activity with methanol or formic acid as substrate Pyrococcus furiosus formate + 2 H+ + reduced benzyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.7.B2 formaldehyde ferredoxin oxidoreductase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.B2 80
-
 assay at Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.7.B2 14
-
 formaldehyde pH 8.4, 20°C Pyrococcus furiosus
1.2.7.B2 54
-
 formaldehyde pH 8.4, 80°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.B2 8.4
-
 assay at Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.7.B2 560
-
 formaldehyde pH 8.4, 20°C Pyrococcus furiosus
1.2.7.B2 1636
-
 formaldehyde pH 8.4, 80°C Pyrococcus furiosus