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Literature summary extracted from

  • Ye, S.; Wu, X.; Wei, L.; Tang, D.; Sun, P.; Bartlam, M.; Rao, Z.
    An insight into the mechanism of human cysteine dioxygenase. Key roles of the thioether-bonded tyrosine-cysteine cofactor (2007), J. Biol. Chem., 282, 3391-3402.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.20 cloned into pGEX-6p-1 expression vector, expressed in Escherichia coli Bl21(DE3), glutathione S-transferase-fused CDO is purified and GTS tag is removed by cleavage with PreScission Protease Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.20 co-crystallization of L-cysteine and purified wild-type cysteine dioxygenase by the hanging drop, vapor-diffusion method at 16°C, the crystals grow as rods up to 0.2 x 0.2 x 0.8 mm in 1 week. Refinement of the crystal stucture leads to a final model with a crystallographic R-factor of 18.1% and a free R-factor of 21.5% at 2.7 A resolution. Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.20 C164A mutation of C164 shows a about 20% abatement of enzymatic activity Homo sapiens
1.13.11.20 C164S mutation of C164 shows a about 20% abatement of enzymatic activity Homo sapiens
1.13.11.20 C93A C93 mutation reduces activity to 50%, zinc content of about 45%, specific activity of Cys-93 mutants is proportional to the measured iron content. Homo sapiens
1.13.11.20 C93S C93 mutation reduces activity to 50%, zinc content of about 45%, specific activity of Cys-93 mutants is proportional to the measured iron content. Homo sapiens
1.13.11.20 R60A similar results as R60Q, R60 mutation reduces activity to 30% Homo sapiens
1.13.11.20 R60Q R60 mutation reduces activity to 30% Homo sapiens
1.13.11.20 Y157F in the gel-filtration chromatography Y157F shows an additional peak with an estimated molecular weight equivalent to a cysteine dioxygenase dimer. The results for monomer and dimer are similar. Activity reduced to 5% of the wild type activity. Zinc content of about 45% Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.20 EDTA an EDTA:cysteine dioxygenase molar ratio of about 1000:1 abolish`s cysteine oxidase activity Homo sapiens
1.13.11.20 homocysteine cysteine dioxygenase activity is reduced by 50% only when the molar ratio of homocysteine:cysteine dioxygenase reach about 30000:1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.20 3.1
-
 L-cysteine under conditions where the second substrate oxygen is saturated Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.20 Fe2+ purified human cystein dioxygenase yields a iron incorporation of about 68% Homo sapiens
1.13.11.20 Fe2+ unsaturated distored tetragonal bipyramidal ferrous center with a vacant coordination site Homo sapiens
1.13.11.20 Mg purified human cystein dioxygenase yields trace amounts of magnesium about 0.41% Homo sapiens
1.13.11.20 Mn purified human cystein dioxygenase yields trace amounts of manganese about 0.25% Homo sapiens
1.13.11.20 Zn2+ purified human cystein dioxygenase yields a zinc incorporation of about 18.1% Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.20 Homo sapiens Q16878
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.20 standard glutathione S-transferase fusion protein purification protocol, gel-filtration chromatography after removing the glutathione S-transferase tag Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.20 L-cysteine + O2
-
 Homo sapiens 3-sulfino-L-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.20 CDO
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.20 1.7
-
 L-cysteine determind under saturating oxygen conditions Homo sapiens