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Literature summary extracted from

  • Buhrke, T.; Lenz, O.; Krauss, N.; Friedrich, B.
    Oxygen tolerance of the H2-sensing [NiFe] hydrogenase from Ralstonia eutropha H16 is based on limited access of oxygen to the active site (2005), J. Biol. Chem., 280, 23791-23796.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.99.6 mutant enzymes expressed in Escherichia coli Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
1.12.99.6 F110L 18% of wild-type H2 uptake activity. The loss of activity of the mutant protein originates from reversible oxidative inactivation Cupriavidus necator
1.12.99.6 I62V 6% of wild-type H2 uptake activity. The loss of activity of the mutant protein originates from reversible oxidative inactivation Cupriavidus necator
1.12.99.6 I62V/F110L mutant enzyme shows no H2 uptake activity. The loss of activity of the mutant protein originates from reversible oxidative inactivation Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Cupriavidus necator
-
-
-
1.12.99.6 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.99.6 oxygen tolerance is based on limited access of oxygen to the active site Cupriavidus necator

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6
-
 Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + acceptor
-
 Cupriavidus necator H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + acceptor
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 H+ + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 H2-sensing [NiFe] hydrogenase
-
 Cupriavidus necator