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Literature summary extracted from

  • Vezina, J.; Barriault, D.; Sylvestre, M.
    Family shuffling of soil DNA to change the regiospecificity of Burkholderia xenovorans LB400 biphenyl dioxygenase (2007), J. Bacteriol., 189, 779-788.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39 expressed in Escherichia coli strains DH11S and C41(DE3) Paraburkholderia xenovorans
1.14.12.18 expressed in Escherichia coli Paraburkholderia xenovorans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.18 F370Y lower reactivity toward 2,2-dichlorobiphenyl but unchanged regiospecificity toward this substrate compared to the wild type enzyme Paraburkholderia xenovorans
1.14.12.18 L283S lower reactivity toward 2,2-dichlorobiphenyl but unchanged regiospecificity toward this substrate compared to the wild type enzyme Paraburkholderia xenovorans
1.14.12.18 M237T lower reactivity toward 2,2-dichlorobiphenyl but unchanged regiospecificity toward this substrate compared to the wild type enzyme Paraburkholderia xenovorans
1.14.12.18 S238T lower reactivity toward 2,2-dichlorobiphenyl but unchanged regiospecificity toward this substrate compared to the wild type enzyme Paraburkholderia xenovorans
1.14.12.18 T377N lower reactivity toward 2,2-dichlorobiphenyl but unchanged regiospecificity toward this substrate compared to the wild type enzyme Paraburkholderia xenovorans

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Paraburkholderia xenovorans
-
-
-
1.14.12.18 Paraburkholderia xenovorans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 2,2',3,3'-tetrachlorobiphenyl + O2
-
 Paraburkholderia xenovorans ?
-
 ?
1.13.11.39 2,2'-dichlorobiphenyl + O2
-
 Paraburkholderia xenovorans cis-5,6-dihydro-5,6-dihydroxy-2,2'-dichlorobiphenyl
-
 ?
1.13.11.39 2,3-dihydroxybiphenyl + O2
-
 Paraburkholderia xenovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ir
1.13.11.39 cis-5,6-dihydro-5,6-dihydroxy-2,2'-dichlorobiphenyl + O2
-
 Paraburkholderia xenovorans ?
-
 ?
1.14.12.18 2,2',3,3'-tetrachlorobiphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans 5,6-dihydro-5,6-dihydroxy-2,2',3,3'-tetrachlorobiphenyl + 4,5-dihydro-4,5-dihydroxy-2,2',3,3'-tetrachlorobiphenyl + NAD+
-
 ?
1.14.12.18 2,2',5,5'-tetrachlorobiphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans 3,4-dihydro-3,4-dihydroxy-2,2',5,5'-tetrachlorobiphenyl + NAD+
-
 ?
1.14.12.18 2,2'-dichlorobiphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans 2,3-dihydroxy-2'-chlorobiphenyl + 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl + NAD+ + HCl
-
 ?
1.14.12.18 2,4',5-trichlorobiphenyl + NADH + H+ + O2
-
 Paraburkholderia xenovorans 3,4-dihydro-3,4-dihydroxy-2,5,4'-trichlorobiphenyl + 2',3'-dihydro-2',3'-dihydroxy-2,5,4'-trichlorobiphenyl + NAD+
-
 ?
1.14.12.18 biphenyl + NAD(P)H + O2
-
 Paraburkholderia xenovorans cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
 ?
1.14.12.18 ethylbenzene + NADH + O2
-
 Paraburkholderia xenovorans ?
-
 ?
1.14.12.18 additional information no activity on toluene and benzene Paraburkholderia xenovorans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 2,3-dihydroxybiphenyl dioxygenase
-
 Paraburkholderia xenovorans
1.13.11.39 BphC
-
 Paraburkholderia xenovorans
1.14.12.18 biphenyl dioxygenase
-
 Paraburkholderia xenovorans
1.14.12.18 BPDO
-
 Paraburkholderia xenovorans
1.14.12.18 BphAE
-
 Paraburkholderia xenovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.12.18 37
-
-
 Paraburkholderia xenovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.18 5.5
-
-
 Paraburkholderia xenovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.18 NADH
-
 Paraburkholderia xenovorans
1.14.12.18 NADPH
-
 Paraburkholderia xenovorans