Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dog An, S.; Turan, P.; Dog An, M.; Arslan, O.; Alkan, M.
    Purification and characterization of Ocimum basilicum L. polyphenol oxidase (2005), J. Agric. Food Chem., 53, 10224-10230.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 additional information
-
 additional information kinetics Ocimum basilicum
1.10.3.1 1.62
-
 catechol pH 8.0, 25°C Ocimum basilicum
1.10.3.1 2.77
-
 4-methylcatechol pH 6.0, 25°C Ocimum basilicum
1.10.3.1 3.42
-
 pyrogallol pH 9.0, 25°C Ocimum basilicum

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Ocimum basilicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 native enzyme 11.5fold by (NH4)2SO4 precipitation, dialysis, and L-tyrosine-4-aminobenzoic acid affinity chromatography Ocimum basilicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 59.46
-
 purified enzyme Ocimum basilicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 4-methylcatechol + O2
-
 Ocimum basilicum 4-methyl-1,2-benzoquinone + H2O
-
 ?
1.10.3.1 catechol + 1/2 O2
-
 Ocimum basilicum 1,2-benzoquinone + H2O
-
 ?
1.10.3.1 pyrogallol + O2
-
 Ocimum basilicum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 diphenolase
-
 Ocimum basilicum
1.10.3.1 polyphenol oxidase
-
 Ocimum basilicum
1.10.3.1 PPO
-
 Ocimum basilicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 20
-
 with substrate 4-methylcatechol Ocimum basilicum
1.10.3.1 40
-
 with substrate catechol Ocimum basilicum
1.10.3.1 50
-
 with substrate pyrogallol Ocimum basilicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.1 10 60
-
 Ocimum basilicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 additional information
-
 heat denaturation kinetics Ocimum basilicum
1.10.3.1 55
-
 30 min, purified enzyme, loss of 50% activity Ocimum basilicum
1.10.3.1 75
-
 30 min, purified enzyme, loss of 55%, 80%, and 70% activity with substrates 4-methylcatechol, catechol, and pyrogallol, respectively Ocimum basilicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 6
-
 with substrate 4-methylcatechol Ocimum basilicum
1.10.3.1 8
-
 with substrate catechol Ocimum basilicum
1.10.3.1 9
-
 with substrate pyrogallol Ocimum basilicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.1 4 9 pH-profile Ocimum basilicum