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Literature summary extracted from

  • Granata, A.; Monzani, E.; Bubacco, L.; Casella, L.
    Mechanistic insight into the activity of tyrosinase from variable-temperature studies in an aqueous/organic solvent (2006), Chemistry, 12, 2504-2514.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 Phenylthiourea binding and inhibition mode Streptomyces antibioticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 additional information
-
 additional information kinetics, the activation free energy is dominated by the enthalpic term, the value of which lies in the relatively narrow range of 61 kJmol-1 irrespective of substrate or reaction type as monophenolase, EC 1.14.18.1, or diphenolase Streptomyces antibioticus
1.14.18.1 0.22
-
 4-hydroxyphenylpropionic acid pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 0.28
-
 methyl 3-hydroxy-L-tyrosinate pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 0.38
-
 methyl 3-hydroxy-L-tyrosinate pH 6.8, 25°C, in aqueous Hepes buffer Streptomyces antibioticus
1.14.18.1 0.4
-
 dopamine pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 0.44
-
 4-hydroxyphenylpropionic acid pH 6.8, 25°C, in aqueous Hepes buffer Streptomyces antibioticus
1.14.18.1 0.51
-
 tyramine pH 6.8, 25°C, in aqueous Hepes buffer Streptomyces antibioticus
1.14.18.1 0.66
-
 tyramine pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 0.8
-
 L-Dopa pH 6.8, 25°C, in aqueous Hepes buffer Streptomyces antibioticus
1.14.18.1 0.82
-
 methyl 3-hydroxy-L-tyrosinate pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 0.93
-
 dopamine pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 2.1
-
 L-Dopa pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 2.1
-
 tyramine pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 2.2
-
 dopamine pH 6.8, 25°C, in aqueous Hepes buffer Streptomyces antibioticus
1.14.18.1 3.7
-
 L-Dopa pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 6.7
-
 4-hydroxyphenylpropionic acid pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 Cu2+ dependent on, type-3 copper enzyme Streptomyces antibioticus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Streptomyces antibioticus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.18.1 L-tyrosine + O2 = dopaquinone + H2O the active site of the enzyme contains a pair of antiferromagnetically coupled Cu2+ ions in the met form, and during turnover both the reduced and the oxy forms are cyclically produced, the rate-limiting step is associated with cleavage of the peroxide O-O bond, reaction and kinetic mechanism Streptomyces antibioticus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 4-hydroxyphenylpropionic acid + O2
-
 Streptomyces antibioticus ?
-
 ?
1.14.18.1 dopamine + O2
-
 Streptomyces antibioticus ?
-
 ?
1.14.18.1 L-DOPA + O2
-
 Streptomyces antibioticus ?
-
 ?
1.14.18.1 L-tyrosine methyl ester + O2 + AH2
-
 Streptomyces antibioticus L-dopa methyl ester + H2O + A
-
 ?
1.14.18.1 methyl 3-hydroxy-L-tyrosinate + O2
-
 Streptomyces antibioticus ?
-
 r
1.14.18.1 additional information activity with phenolic and diphenolic substrates, also performing the reaction of tyrosinase, a ortho-hydroxylation of monophenols, EC 1.14.18.1, and the oxidation of catechols to ortho-quinones, the diphenolase activity, EC 1.10.3.1, overview Streptomyces antibioticus ?
-
 ?
1.14.18.1 tyramine + O2
-
 Streptomyces antibioticus 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 diphenolase
-
 Streptomyces antibioticus
1.14.18.1 More cf. EC 1.10.3.1 Streptomyces antibioticus
1.14.18.1 tyrosinase
-
 Streptomyces antibioticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 10 25 assay at Streptomyces antibioticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.1 1.7
-
 4-hydroxyphenylpropionic acid pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.10.3.1 1.9
-
 tyramine pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.10.3.1 2.24
-
 methyl 3-hydroxy-L-tyrosinate pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.10.3.1 3.4
-
 methyl 3-hydroxy-L-tyrosinate pH 6.8, 25°C, in aqueous buffer Streptomyces antibioticus
1.10.3.1 7.4
-
 tyramine pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.10.3.1 8.9
-
 methyl 3-hydroxy-L-tyrosinate pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.10.3.1 25.9
-
 tyramine pH 6.8, 25°C, in aqueous buffer Streptomyces antibioticus
1.10.3.1 29.9
-
 dopamine pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.10.3.1 42
-
 L-Dopa pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.10.3.1 43
-
 4-hydroxyphenylpropionic acid pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.10.3.1 66.7
-
 4-hydroxyphenylpropionic acid pH 6.8, 25°C, in aqueous buffer Streptomyces antibioticus
1.10.3.1 107.4
-
 L-Dopa pH 6.8, 25°C, in aqueous buffer Streptomyces antibioticus
1.10.3.1 133.2
-
 dopamine pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.10.3.1 141.1
-
 L-Dopa pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.10.3.1 439
-
 dopamine pH 6.8, 25°C, in aqueous buffer Streptomyces antibioticus
1.14.18.1 0.04
-
 L-Dopa pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 1.9
-
 tyramine pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 2.24
-
 methyl 3-hydroxy-L-tyrosinate pH 6.8, 10°C, in methanol–glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 3.4
-
 methyl 3-hydroxy-L-tyrosinate pH 6.8, 25°C, in aqueous buffer Streptomyces antibioticus
1.14.18.1 7.4
-
 tyramine pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 8.9
-
 methyl 3-hydroxy-L-tyrosinate pH 6.8, 25°C, in methanol–glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 25.9
-
 tyramine pH 6.8, 25°C, in aqueous buffer Streptomyces antibioticus
1.14.18.1 29.9
-
 dopamine pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 42
-
 L-Dopa pH 6.8, 10°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 43
-
 4-hydroxyphenylpropionic acid pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 66.7
-
 4-hydroxyphenylpropionic acid pH 6.8, 25°C, in aqueous buffer Streptomyces antibioticus
1.14.18.1 107.4
-
 L-Dopa pH 6.8, 25°C, in aqueous buffer Streptomyces antibioticus
1.14.18.1 133.2
-
 dopamine pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 141.1
-
 L-Dopa pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 433
-
 dopamine pH 6.8, 25°C, in methanol-glycerol cryosolvent Streptomyces antibioticus
1.14.18.1 439
-
 dopamine pH 6.8, 25°C, in aqueous buffer Streptomyces antibioticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6.8
-
 assay at Streptomyces antibioticus