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Literature summary extracted from

  • Murugan, R.; Mazumdar, S.
    Structure and redox properties of the haem centre in the C357M mutant of cytochrome P450cam (2005), ChemBioChem, 6, 1204-1211.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.1 overexpression in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 C357M site-directed mutagenesis, comparison of the mutant structure to the wild-type one Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.1 Fe2+ heme iron, heme centre tertiary structure and redox potential analysis of wild-type and C357M mutant enzymes, overview Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin Pseudomonas putida
-
 (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida
-
 gene camC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-camphor + O2 + reduced putidaredoxin
-
 Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.1 More secondary structure analysis of wild-type and mutant C357M enzymes, overview Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 Cyt P450cam
-
 Pseudomonas putida
1.14.15.1 cytochrome p450cam
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.1 7.4
-
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.1 putidaredoxin
-
 Pseudomonas putida