EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.167 | biotechnology | optimizing the semi-biosynthetic method in bacteria potentially provides a practical means of developing commercial-scale production of baccatin III analogs, which serve as key intermediates in the semi-synthesis of second-generation taxols | Taxus sp. |
2.3.1.167 | synthesis | bioengineered Escherichia coli cells synthesize nonnatural 10-deacetyl-10-acylbaccatin III derivatives as potential precursors of second-generation Taxol derivatives using the enzyme in concert with acyl-CoA transferases, determination of optimal alkanoic acid concentration for in vivo production of baccatin III analogues, overview | Taxus sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.167 | gene dbat, functional expression in Escherichia coli strain JM109, co-expression with acetyl-CoA synthase, gene acs, EC 6.2.1.1, or with acetoacetate:acetyl-CoA CoA-transferase, genes atoAD of the ato operon, EC 2.8.3.8, expressed from and regulated by genes encoded on the bacterial chromosome, overview | Taxus sp. |
2.3.1.167 | into the vector pCWori+ for expression in Escherichia coli JM109 and BL21DE3 cells | Taxus sp. |
6.2.1.1 | 10-deacetylbaccatin III 10beta-O-acetyltransferase (DBAT) from Taxus can catalyze the transfer of acetyl, propionyl or n-butyryl from CoA to the C10-hydroxyl of 10-deacetylbaccatin III. Escherichia coli JM109 are transformed to recombinantly express dbat, and this enzyme function is coupled to that of acetyl-CoA synthase expressed from and regulated by genes encoded on the bacterial chromosome | Taxus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.167 | acetyl-CoA + 10-deacetylbaccatin III | Taxus sp. | - |
CoA + baccatin III | - |
? | |
2.3.1.167 | butyryl-CoA + 10-deacetylbaccatin III | Taxus sp. | - |
CoA + 10-deacetyl-10-butyrylbaccatin III | - |
? | |
2.3.1.167 | propionyl-CoA + 10-deacetylbaccatin III | Taxus sp. | - |
CoA + 10-deacetyl-10-propionylbaccatin III | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.167 | Taxus sp. | - |
- |
- |
2.3.1.167 | Taxus sp. | - |
Taxus | - |
6.2.1.1 | Taxus sp. | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.167 | acetyl-CoA + 10-deacetylbaccatin III | - |
Taxus sp. | CoA + baccatin III | - |
? | |
2.3.1.167 | butyryl-CoA + 10-deacetylbaccatin III | - |
Taxus sp. | CoA + 10-deacetyl-10-butyrylbaccatin III | - |
? | |
2.3.1.167 | additional information | DBAT catalyzes the transfer of acetyl, propionyl or n-butyryl from CoA to the C10-hydroxyl of 10-deacetylbaccatin III | Taxus sp. | ? | - |
? | |
2.3.1.167 | n-butyryl-CoA + 10-deacetylbaccatin III | - |
Taxus sp. | CoA + 10-deacetyl-10-butyrylbaccatin III | - |
? | |
2.3.1.167 | propionyl-CoA + 10-deacetylbaccatin III | - |
Taxus sp. | CoA + 10-deacetyl-10-propionylbaccatin III | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.167 | 10-deacetylbaccatin III 10-O-acetyltransferase | - |
Taxus sp. |
2.3.1.167 | 10-deacetylbaccatin III 10beta-O-acetyltransferase | - |
Taxus sp. |
2.3.1.167 | DBAT | - |
Taxus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.167 | acetyl-CoA | - |
Taxus sp. |