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Literature summary extracted from

  • Jun, S.; Fushinobu, S.; Nojiri, H.; Omori, T.; Shoun, H.; Wakagi, T.
    Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01 (2006), Biochim. Biophys. Acta, 1764, 1159-1166.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.7.1.9 A129V mutant, at 1.65 A resolution, crystals of the A129V mutant isomorphous with the S103A crystals Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
3.7.1.9 A129V improved catalytic efficiency than the wild type enzyme, possesses a more appropiate binding pocket for substrates with smaller C6 substituents Pseudomonas fluorescens
3.7.1.9 A129V/I199V/V227I combination of mutations incompatible for correct folding Pseudomonas fluorescens
3.7.1.9 F104M mutation does not have any significant effect on enzyme characteristics Pseudomonas fluorescens
3.7.1.9 I199V improved catalytic efficiency than the wild type enzyme Pseudomonas fluorescens
3.7.1.9 I199V/V2271 combination of mutations incompatible for correct folding Pseudomonas fluorescens
3.7.1.9 S34A lower Km and turnover rates compared to wild-type Pseudomonas fluorescens
3.7.1.9 S34G lower Km and turnover rates compared to wild-type Pseudomonas fluorescens
3.7.1.9 V227I improved catalytic efficiency than the wild type enzyme Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.9 0.00045
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant I199V Pseudomonas fluorescens
3.7.1.9 0.0008
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant S34G Pseudomonas fluorescens
3.7.1.9 0.0014
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant I199V Pseudomonas fluorescens
3.7.1.9 0.0017
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
3.7.1.9 0.0018
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129V Pseudomonas fluorescens
3.7.1.9 0.002
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant V227I Pseudomonas fluorescens
3.7.1.9 0.0022
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate wild-type Pseudomonas fluorescens
3.7.1.9 0.0023
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant I199V Pseudomonas fluorescens
3.7.1.9 0.0024
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
3.7.1.9 0.0025
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant S34A Pseudomonas fluorescens
3.7.1.9 0.0026
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant S34G Pseudomonas fluorescens
3.7.1.9 0.0027
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant F104M Pseudomonas fluorescens
3.7.1.9 0.0028
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant V227I Pseudomonas fluorescens
3.7.1.9 0.0031
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant S34A Pseudomonas fluorescens
3.7.1.9 0.0035
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
3.7.1.9 0.0035
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant I199V Pseudomonas fluorescens
3.7.1.9 0.0039
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant F104M Pseudomonas fluorescens
3.7.1.9 0.004
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant F104M Pseudomonas fluorescens
3.7.1.9 0.0042
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant S34G Pseudomonas fluorescens
3.7.1.9 0.0048
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
3.7.1.9 0.0049
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant V227I Pseudomonas fluorescens
3.7.1.9 0.0049
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant V227I Pseudomonas fluorescens
3.7.1.9 0.005
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
3.7.1.9 0.005
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant S34A Pseudomonas fluorescens
3.7.1.9 0.0055
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
3.7.1.9 0.0056
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129V Pseudomonas fluorescens
3.7.1.9 0.0058
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
3.7.1.9 0.0078
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate wild-type Pseudomonas fluorescens
3.7.1.9 0.0089
-
 2-hydroxy-6-oxohepta-2,4-dienoate wild-type Pseudomonas fluorescens
3.7.1.9 0.0098
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129V Pseudomonas fluorescens
3.7.1.9 0.01
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
3.7.1.9 0.01
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129V Pseudomonas fluorescens
3.7.1.9 0.011
-
 2-hydroxy-6-oxoocta-2,4-dienoate wild-type Pseudomonas fluorescens
3.7.1.9 0.015
-
 2-hydroxy-6-oxoocta-2,4-dienoate
-
 Pseudomonas putida
3.7.1.9 0.017
-
 2-hydroxy-6-oxohepta-2,4-dienoate
-
 Pseudomonas putida
3.7.1.9 0.022
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate
-
 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.9 Pseudomonas fluorescens
-
 IP01
-
3.7.1.9 Pseudomonas fluorescens IP01
-
 IP01
-
3.7.1.9 Pseudomonas putida
-
 F1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.9 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.7.1.9 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Pseudomonas fluorescens IP01 ?
-
 ?
3.7.1.9 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.7.1.9 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens IP01 ?
-
 ?
3.7.1.9 2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.7.1.9 2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 2-hydroxy-6-oxohepta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens IP01 ?
-
 ?
3.7.1.9 2-hydroxy-6-oxoocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.7.1.9 2-hydroxy-6-oxoocta-2,4-dienoate + H2O
-
 Pseudomonas putida ?
-
 ?
3.7.1.9 2-hydroxy-6-oxoocta-2,4-dienoate + H2O
-
 Pseudomonas fluorescens IP01 ?
-
 ?
3.7.1.9 additional information can not hydrolyze 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas fluorescens ?
-
 ?
3.7.1.9 additional information can not hydrolyze 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas putida ?
-
 ?
3.7.1.9 additional information can not hydrolyze 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate Pseudomonas fluorescens IP01 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.7.1.9 CumD
-
 Pseudomonas fluorescens
3.7.1.9 meta-cleavage product hydrolase
-
 Pseudomonas fluorescens
3.7.1.9 meta-cleavage product hydrolase
-
 Pseudomonas putida
3.7.1.9 TodF
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.9 0.002
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
3.7.1.9 0.01
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
3.7.1.9 0.01
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant A129V Pseudomonas fluorescens
3.7.1.9 0.02
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant I199V Pseudomonas fluorescens
3.7.1.9 0.02
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate mutant V227I Pseudomonas fluorescens
3.7.1.9 0.037
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate wild-type Pseudomonas fluorescens
3.7.1.9 0.3
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant S34A Pseudomonas fluorescens
3.7.1.9 0.41
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate
-
 Pseudomonas putida
3.7.1.9 0.73
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
3.7.1.9 1.3
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant S34A Pseudomonas fluorescens
3.7.1.9 1.5
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant S34G Pseudomonas fluorescens
3.7.1.9 3.3
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
3.7.1.9 4.55
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
3.7.1.9 6.4
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant F104M Pseudomonas fluorescens
3.7.1.9 6.5
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant F104M Pseudomonas fluorescens
3.7.1.9 6.7
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant S34A Pseudomonas fluorescens
3.7.1.9 8.1
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant S34G Pseudomonas fluorescens
3.7.1.9 8.4
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant S34G Pseudomonas fluorescens
3.7.1.9 14
-
 2-hydroxy-6-oxoocta-2,4-dienoate
-
 Pseudomonas putida
3.7.1.9 15.4
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant F104M Pseudomonas fluorescens
3.7.1.9 19
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
3.7.1.9 19.5
-
 2-hydroxy-6-oxohepta-2,4-dienoate wild-type Pseudomonas fluorescens
3.7.1.9 20
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant V227I Pseudomonas fluorescens
3.7.1.9 23.1
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate wild-type Pseudomonas fluorescens
3.7.1.9 24
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
3.7.1.9 25
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant A129V Pseudomonas fluorescens
3.7.1.9 29
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129/V227I Pseudomonas fluorescens
3.7.1.9 29
-
 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate mutant I199V Pseudomonas fluorescens
3.7.1.9 35
-
 2-hydroxy-6-oxohepta-2,4-dienoate
-
 Pseudomonas putida
3.7.1.9 35
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129/I199V Pseudomonas fluorescens
3.7.1.9 37
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant I199V Pseudomonas fluorescens
3.7.1.9 38
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant I199V Pseudomonas fluorescens
3.7.1.9 42.4
-
 2-hydroxy-6-oxoocta-2,4-dienoate wild-type Pseudomonas fluorescens
3.7.1.9 52
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant V227I Pseudomonas fluorescens
3.7.1.9 75
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant A129V Pseudomonas fluorescens
3.7.1.9 78
-
 2-hydroxy-6-oxoocta-2,4-dienoate mutant V227I Pseudomonas fluorescens
3.7.1.9 81
-
 2-hydroxy-6-oxohepta-2,4-dienoate mutant A129V Pseudomonas fluorescens