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Literature summary extracted from

  • Morar, M.; Anand, R.; Hoskins, A.A.; Stubbe, J.; Ealick, S.E.
    Complexed structures of formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima describe a novel ATP binding protein superfamily (2006), Biochemistry, 45, 14880-14895.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.5.3 hanging drop method, enzyme in complex with formylglycinamide ribonucleotide, mutant enzyme H72A in complex with beta,gamma-methylene adenosine 5'-triphosphate, mutant enzyme H72A in complex with ADP, enzyme in complex with formylglycinamide ribonucleotide and beta,gamma-methylene adenosine 5'-triphosphate, enzyme in complex with ATP Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.3 H32A no synthesis of formylglycinamidine ribonucleotide detected Thermotoga maritima
6.3.5.3 H32Q no synthesis of formylglycinamidine ribonucleotide detected Thermotoga maritima
6.3.5.3 H72A mutant enzyme displays 1/20 of the wild type activity with a strongly increased Km for formylglycinamidine ribonucleotide Thermotoga maritima
6.3.5.3 H72Q mutant enzyme displayed 1/200 of the wild type activity Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.3 Thermotoga maritima Q9X0X3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.3 wild-type and mutant enzymes expression in Escherichia coli Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.3 ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O fourth step of the purine biosynthetic pathway Thermotoga maritima ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.3 FGAR-AT
-
 Thermotoga maritima
6.3.5.3 TmPurL
-
 Thermotoga maritima