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Literature summary extracted from

  • Coe, D.M.; Viola, R.E.
    Assessing the roles of essential functional groups in the mechanism of homoserine succinyltransferase (2007), Arch. Biochem. Biophys., 461, 211-218.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.46 metA, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.46 C142A site-directed mutagenesis, the mutant is inactive Escherichia coli
2.3.1.46 C142S site-directed mutagenesis, the mutant is almost inactive Escherichia coli
2.3.1.46 E237A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Escherichia coli
2.3.1.46 E237D site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Escherichia coli
2.3.1.46 E246A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Escherichia coli
2.3.1.46 E246D site-directed mutagenesis, the mutant shows reduced catalytic efficiency with L-homoserine, but increased with succinyl-CoA compared to the wild-type enzyme Escherichia coli
2.3.1.46 H235N site-directed mutagenesis, the mutant is almost inactive Escherichia coli
2.3.1.46 K156L site-directed mutagenesis, the mutant is inactive Escherichia coli
2.3.1.46 K45A/K46A site-directed mutagenesis, the mutant is inactive Escherichia coli
2.3.1.46 K45L site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Escherichia coli
2.3.1.46 K46L site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Escherichia coli
2.3.1.46 R193A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Escherichia coli
2.3.1.46 R193A/E246A site-directed mutagenesis, the mutant shows highly reduced catalytic efficiency with L-homoserine and reduced activity with succinyl-CoA compared to the wild-type enzyme Escherichia coli
2.3.1.46 R193K site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Escherichia coli
2.3.1.46 R249A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Escherichia coli
2.3.1.46 R249K site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Escherichia coli
2.3.1.46 Y238F site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Escherichia coli
2.3.1.46 Y238F/E246A site-directed mutagenesis, the mutant shows highly reduced catalytic efficiency with L-homoserine, but increased with succinyl-CoA compared to the wild-type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.46 additional information
-
 additional information steady-state kinetics and thermodynamics of wild-type and mutant enzymes, overview Escherichia coli
2.3.1.46 0.043
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant E246D Escherichia coli
2.3.1.46 0.044
-
 L-homoserine pH 7.5, 25°C, recombinant mutant K45L Escherichia coli
2.3.1.46 0.05
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant Y238F/E246A Escherichia coli
2.3.1.46 0.094
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant E246A Escherichia coli
2.3.1.46 0.15
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant E237A Escherichia coli
2.3.1.46 0.2
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant R193A/E246A Escherichia coli
2.3.1.46 0.23
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant R249A Escherichia coli
2.3.1.46 0.24
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant E237D Escherichia coli
2.3.1.46 0.28
-
 succinyl-CoA pH 7.5, 25°C, recombinant wild-type enzyme Escherichia coli
2.3.1.46 0.31
-
 L-homoserine pH 7.5, 25°C, recombinant mutant R193K Escherichia coli
2.3.1.46 0.31
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant R249K Escherichia coli
2.3.1.46 0.35
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant Y238F Escherichia coli
2.3.1.46 0.36
-
 L-homoserine pH 7.5, 25°C, recombinant mutant E237D Escherichia coli
2.3.1.46 0.38
-
 L-homoserine pH 7.5, 25°C, recombinant wild-type enzyme Escherichia coli
2.3.1.46 0.4
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant R193K Escherichia coli
2.3.1.46 0.43
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant R193A Escherichia coli
2.3.1.46 1.1
-
 L-homoserine pH 7.5, 25°C, recombinant mutant E237A Escherichia coli
2.3.1.46 1.1
-
 L-homoserine pH 7.5, 25°C, recombinant mutant R193A Escherichia coli
2.3.1.46 1.15
-
 L-homoserine pH 7.5, 25°C, recombinant mutant R249A Escherichia coli
2.3.1.46 1.51
-
 L-homoserine pH 7.5, 25°C, recombinant mutant R249K Escherichia coli
2.3.1.46 1.78
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant K46L Escherichia coli
2.3.1.46 2
-
 L-homoserine pH 7.5, 25°C, recombinant mutant Y238F Escherichia coli
2.3.1.46 2.26
-
 L-homoserine pH 7.5, 25°C, recombinant mutant K46L Escherichia coli
2.3.1.46 2.9
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant K45L Escherichia coli
2.3.1.46 9.1
-
 L-homoserine pH 7.5, 25°C, recombinant mutant E246D Escherichia coli
2.3.1.46 59.1
-
 L-homoserine pH 7.5, 25°C, recombinant mutant E246A Escherichia coli
2.3.1.46 93.8
-
 L-homoserine pH 7.5, 25°C, recombinant mutant R193A/E246A Escherichia coli
2.3.1.46 95.5
-
 L-homoserine pH 7.5, 25°C, recombinant mutant Y238F/E246A Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.46 succinyl-CoA + L-homoserine Escherichia coli
-
 CoA + O-succinyl-L-homoserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.46 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.46 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by 2 different steps of anion exchange chromatography Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.46 succinyl-CoA + L-homoserine = CoA + O-succinyl-L-homoserine ping pong catalytic reaction mechanism, roles of essential functional groups, the catalytic triad is formed by the nucleophile Cys142, the essential base His235, and Glu237, required for proper orientation of His235, Lys46 is required for interaction with succinyl-CoA, and Arg193 is involved in binding of L-Homoserine Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.46 additional information
-
 recombinant wild-type and mutant enzymes Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.46 succinyl-CoA + L-homoserine
-
 Escherichia coli CoA + O-succinyl-L-homoserine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.46 homoserine succinyltransferase
-
 Escherichia coli
2.3.1.46 HST
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.46 25
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.46 0.8
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant E237A Escherichia coli
2.3.1.46 1.2
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant R193A/E246A Escherichia coli
2.3.1.46 1.26
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant K46L Escherichia coli
2.3.1.46 2 3.7 succinyl-CoA pH 7.5, 25°C, recombinant mutant R249K Escherichia coli
2.3.1.46 3.3
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant R193A Escherichia coli
2.3.1.46 3.4
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant R193K Escherichia coli
2.3.1.46 6.7
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant E237D Escherichia coli
2.3.1.46 6.9
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant E246A Escherichia coli
2.3.1.46 8.9
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant Y238F/E246A Escherichia coli
2.3.1.46 10
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant R249A Escherichia coli
2.3.1.46 13
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant Y238F Escherichia coli
2.3.1.46 15
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant E246D Escherichia coli
2.3.1.46 25.7
-
 succinyl-CoA pH 7.5, 25°C, recombinant wild-type enzyme Escherichia coli
2.3.1.46 40.1
-
 succinyl-CoA pH 7.5, 25°C, recombinant mutant K45L Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.46 7.5
-
 assay at Escherichia coli