EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.87 | Mg2+ | - |
Pseudomonas sp. | |
3.5.1.87 | Zn2+ | - |
Pseudomonas sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.87 | NCC amidohydrolase gene is cloned into DH5alpha and pet15b vectors for protein expression in Escherichia coli | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.87 | EDTA | - |
Pseudomonas sp. | |
3.5.1.87 | Hg2+ | - |
Pseudomonas sp. | |
3.5.1.87 | iodoacetic acid | IAA | Pseudomonas sp. | |
3.5.1.87 | N-ethyl-maleimide | NEM | Pseudomonas sp. | |
3.5.1.87 | p-chloromercuribenzoic acid | PCMB | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.87 | Mn2+ | - |
Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.87 | 45000 | - |
monomer, 12,5% SDS-PAGE | Pseudomonas sp. |
3.5.1.87 | 170000 | - |
native enzyme, tetramer, estimated by Sephacryl S-300 gel filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.87 | N-carbamoyl-L-cysteine + H2O | Pseudomonas sp. | - |
L-cysteine + CO2 + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.87 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.87 | anion-exchange chromatography, hydrophobic interaction chromatography hydroxyapatite chromatography used for purification of native expressed protein | Pseudomonas sp. |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.5.1.87 | protein from inclusion bodies is solubilized, recovered and purified by metal affinity chromatography | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.87 | 15.9 | - |
protein recovered from inclusion bodies | Pseudomonas sp. |
3.5.1.87 | 23.2 | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.87 | N-carbamoyl-DL-aspartic acid + H2O | relative activity 7.81% | Pseudomonas sp. | DL-aspartic acid + CO2 + NH3 | - |
? | |
3.5.1.87 | N-carbamoyl-DL-cysteine + H2O | relative activity 119% | Pseudomonas sp. | DL-cysteine + CO2 + NH3 | - |
? | |
3.5.1.87 | N-carbamoyl-DL-serine + H2O | relative activity 37.8% | Pseudomonas sp. | DL-serine + CO2 + NH3 | - |
? | |
3.5.1.87 | N-carbamoyl-glycine + H2O | relative activity 72.4% | Pseudomonas sp. | glycine + CO2 + NH3 | - |
? | |
3.5.1.87 | N-carbamoyl-L-alanine + H2O | relative activity 41.2% | Pseudomonas sp. | L-alanine + CO2 + NH3 | - |
? | |
3.5.1.87 | N-carbamoyl-L-cysteine + H2O | - |
Pseudomonas sp. | L-cysteine + CO2 + NH3 | - |
? | |
3.5.1.87 | N-carbamoyl-L-cysteine + H2O | relative activity 100% | Pseudomonas sp. | L-cysteine + CO2 + NH3 | - |
? | |
3.5.1.87 | N-carbamoyl-L-methionine + H2O | relative activity 44.4% | Pseudomonas sp. | L-methionine + CO2 + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.87 | tetramer | - |
Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.87 | N-carbamoyl-L-amino-acid amidohydrolase | - |
Pseudomonas sp. |
3.5.1.87 | N-carbamoyl-L-amino-acid hydrolase | - |
Pseudomonas sp. |
3.5.1.87 | N-carbamoyl-L-cysteine-acid amidohydrolase | - |
Pseudomonas sp. |
3.5.1.87 | NCC amidohydrolase | - |
Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.87 | 50 | - |
activity assay | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.87 | 9 | - |
activity assay | Pseudomonas sp. |