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Literature summary extracted from

  • Pozdnyakova, N.N.; Turkovskaya, O.V.; Yudina, E.N.; Rodakiewicz-Nowak, Y.
    Yellow laccase from the fungus Pleurotus ostreatus D1: purification and characterization (2006), Appl. Biochem. Microbiol., 42, 56-61.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2-mercaptoethanol complete inhibition at 0.1 mM Pleurotus ostreatus
1.10.3.2 EDTA 60% inhibition at 100 mM Pleurotus ostreatus
1.10.3.2 SDS complete inhibition at 140 mM Pleurotus ostreatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0087
-
 syringaldazine pH 7.0, 25°C Pleurotus ostreatus
1.10.3.2 0.11
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, 25°C Pleurotus ostreatus
1.10.3.2 0.43
-
 2,6-dimethoxyphenol pH 7.0, 25°C Pleurotus ostreatus
1.10.3.2 3.65
-
 o-benzenediol pH 7.0, 25°C Pleurotus ostreatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Pleurotus ostreatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ copper-containing enzyme, type I copper atoms Pleurotus ostreatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 64000
-
 1 * 64000, SDS-PAGE Pleurotus ostreatus
1.10.3.2 66000
-
 gel filtration Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Pleurotus ostreatus
-
-
-
1.10.3.2 Pleurotus ostreatus D1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native extracellular enzyme by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration Pleurotus ostreatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 additional information solid-phase culture, natural substrate are sunflower-seed shells Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 additional information
-
-
 Pleurotus ostreatus
1.10.3.2 5.8
-
 purified enzyme, substrate syringaldazine Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Pleurotus ostreatus ?
-
 ?
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Pleurotus ostreatus D1 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Pleurotus ostreatus 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Pleurotus ostreatus D1 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
1.10.3.2 additional information no activity with tyrosine and hydroquinone, the enzyme in absence of a mediator oxidizes anthracene to anthraquinone to 95% Pleurotus ostreatus ?
-
 ?
1.10.3.2 additional information no activity with tyrosine and hydroquinone, the enzyme in absence of a mediator oxidizes anthracene to anthraquinone to 95% Pleurotus ostreatus D1 ?
-
 ?
1.10.3.2 pyrocatechol + O2
-
 Pleurotus ostreatus ?
-
 ?
1.10.3.2 pyrocatechol + O2
-
 Pleurotus ostreatus D1 ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Pleurotus ostreatus ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Pleurotus ostreatus D1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 64000, SDS-PAGE Pleurotus ostreatus

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 yellow laccase
-
 Pleurotus ostreatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 25
-
 assay at Pleurotus ostreatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.04 1.97 2,6-dimethoxyphenol pH 7.0, 25°C Pleurotus ostreatus
1.10.3.2 2 8 o-benzenediol pH 7.0, 25°C Pleurotus ostreatus
1.10.3.2 6.1
-
 syringaldazine pH 7.0, 25°C Pleurotus ostreatus
1.10.3.2 8.94
-
 2,6-dimethoxyphenol pH 7.0, 25°C Pleurotus ostreatus
1.10.3.2 11.7
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, 25°C Pleurotus ostreatus
1.10.3.2 21.98
-
 o-benzenediol pH 7.0, 25°C Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4 8 dependent on the substrate, overview Pleurotus ostreatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2 8.8
-
 Pleurotus ostreatus