Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Seetharamappa, J.; Oke, M.; Liu, H.; McMahon, S.A.; Johnson, K.A.; Carter, L.; Dorward, M.; Zawadzki, M.; Overton, I.M.; van Niekirk, C.A.; Graham, S.; Botting, C.H.; Taylor, G.L.; White, M.F.; Barton, G.J.; Coote, P.J.; Naismith, J.H.
    Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5-phosphate-dependent aminotransferase (2007), Acta Crystallogr. Sect. F, F63, 452-456.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.1 expressed in Escherichia coli BL21 (DE3) cells Staphylococcus aureus
2.6.1.5 expressed in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.1 hanging drop vapour diffusion and sitting drop vapour diffusion using 23.6% (w/v) PEG 4000, 0.1 M HEPES pH 8.0 Staphylococcus aureus
2.6.1.5 hanging drop vapour diffusion method using 23.6% (w/v) PEG 4000, 0.1 M HEPES, pH 8.0 Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 48170
-
-
 Staphylococcus aureus
2.6.1.5 48170
-
 X-ray crystallography Staphylococcus aureus
2.6.1.5 50000
-
 SDS-PAGE Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Staphylococcus aureus
-
-
-
2.6.1.5 Staphylococcus aureus A0A163U8E7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.1 nickel Sepharose 6 fast flow chromatography and S-75 gel filtration Staphylococcus aureus
2.6.1.5 nickel Sepharose 6 fast flow chromatography, HiPrep 26/10 desalting column chromatography, and S-75 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate
-
 Staphylococcus aureus oxaloacetate + L-glutamate
-
 ?
2.6.1.5 L-tyrosine + 2-oxoglutarate
-
 Staphylococcus aureus 4-hydroxyphenylpyruvate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.1 dimer
-
 Staphylococcus aureus
2.6.1.5 dimer S-75 gel filtration Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.1 aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase
-
 Staphylococcus aureus
2.6.1.1 Sar2028
-
 Staphylococcus aureus
2.6.1.5 Sar2028
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.5 pyridoxal 5'-phosphate dependent Staphylococcus aureus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.1 Staphylococcus aureus
-
-
 5.25
2.6.1.5 Staphylococcus aureus 2D-PAGE
-
 5.25