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Literature summary extracted from

  • Kataoka, S.; Nakamura, S.; Ohkubo, T.; Ueda, S.; Uchiyama, S.; Kobayashi, Y.; Oda, M.
    Crystallization and preliminary X-ray analysis of the complex of NADH and 3alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831 (2006), Acta Crystallogr. Sect. F, 62, 569-571.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.50 expression in Escherichia coli Pseudomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.50 purified recombinant enzyme in complex with NADH, hanging drop vapour diffusion method and microseeding, 0.002 ml of protein solution with 0.3 mM 3alpha-HSD and 0.4 mM NADH in 10 mM Tris–HCl pH 8.0, are mixed with 0.002 ml reservoir solution optimally containing 0.1 M Tris–HCl, pH 9.0, 0.14 M sodium chloride and 1.4 M ammonium sulfate, 4°C, X-ray diffraction structure determination and analysis at 1.8 A resolution Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 26000
-
 2 * 26000, recombinant enzyme Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50 recombinant enzyme from Escherichia coli Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 additional information the enzyme catalyzes the reversible interconversion of hydroxyl and oxo groups at position 3 of the steroid nucleus Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.50 dimer 2 * 26000, recombinant enzyme Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.50 3alpha-HSD
-
 Pseudomonas sp.
1.1.1.50 3alpha-hydroxysteroid dehydrogenase
-
 Pseudomonas sp.
1.1.1.50 More the enzyme belongs to the short-chain dehydrogenase/reductase, SDR, superfamily Pseudomonas sp.
1.1.1.50 NAD(P)+-3alpha-hydroxysteroid dehydrogenase
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NADH
-
 Pseudomonas sp.