Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Steinbacher, S.; Schiffmann, S.; Bacher, A.; Fischer, M.
    Metal sites in 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with the substrate ribulose 5-phosphate (2004), Acta Crystallogr. Sect. D, 60, 1338-1340.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.99.12 purified enzyme mutant H147S in complex with substrate ribulose 5-phosphate, monoclinic crystal form, X-ray diffraction structure determination and analysis at 1.55-1.7 A resolution Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.12 H147S site-directed mutagenesis, the mutant shows about 10% of wild-type enzyme activity, crystal structure determination with bound substrate Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.12 Ca2+ Ca2+ binding requires the binding of the substrate, binding involves Glu204, Glu97, and Asn207 Methanocaldococcus jannaschii
4.1.99.12 additional information substrate binding structure involving a dimetal center, overview Methanocaldococcus jannaschii
4.1.99.12 Zn2+ bound at His164, binding involves His206, Glu132, and Glu128 Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.12 D-ribulose 5-phosphate Methanocaldococcus jannaschii DHBPS supplies the building blocks for the assembly of the xylene ring of the vitamin B2, riboflavin, all eight C atoms of the xylene moiety are derived from the product of the enzyme formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.12 Methanocaldococcus jannaschii
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.99.12 D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate active site architecture Methanocaldococcus jannaschii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.12 D-ribulose 5-phosphate DHBPS supplies the building blocks for the assembly of the xylene ring of the vitamin B2, riboflavin, all eight C atoms of the xylene moiety are derived from the product of the enzyme Methanocaldococcus jannaschii formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
4.1.99.12 D-ribulose 5-phosphate substrate binding structure involving a dimetal center, overview Methanocaldococcus jannaschii formate + L-3,4-dihydroxy-2-butanone-4-phosphate the enzyme converts ribulose 5-phosphate into 3,4-dihydroxy-2-butanone 4-phosphate, while its C4 atom is released as formate ?

Subunits

EC Number Subunits Comment Organism
4.1.99.12 dimer enzyme mutant H147S, crystal structure Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
4.1.99.12 3,4-dihydroxy-2-butanone 4-phosphate synthase
-
 Methanocaldococcus jannaschii
4.1.99.12 DHBPS
-
 Methanocaldococcus jannaschii