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Literature summary extracted from

  • Wildermuth, M.C.; Fall, R.
    Light-dependent isoprene emission (characterization of a thylakoid-bound isoprene synthase in Salix discolor chloroplasts) (1996), Plant Physiol., 112, 171-182.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.27 additional information light-dependent regulation of the thylakoid membrane-bound enzyme, overview Salix discolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.27 8
-
 dimethylallyl diphosphate pH 7.8 Salix discolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.27 chloroplast
-
 Salix discolor 9507
-
4.2.3.27 additional information study of subcellular localization, overview Salix discolor
-
-
4.2.3.27 soluble soluble enzyme form Salix discolor
-
-
4.2.3.27 thylakoid membrane tightly membrane-bound enzyme form with a stromal facing, accessible to trypsin treatment Salix discolor 42651
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.27 Mg2+ dependent on, optimal at 10-15 mM, activity profile at 0-25 mM MgCl2 Salix discolor
4.2.3.27 Mn2+ can substitute for Mg2+ Salix discolor
4.2.3.27 additional information no effect by Ca2+ Salix discolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Salix discolor light-dependent regulation of the thylakoid membrane-bound enzyme, overview isoprene + diphosphate isoprene is a volatile product emmitted to the atmosphere ir

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Salix discolor
-
 soluble and membrane-bound isozymes
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.27 leaf
-
 Salix discolor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.27 additional information
-
 activity in subcellular fractions during partial purification, overview Salix discolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate
-
 Salix discolor isoprene + diphosphate i.e. 2-methyl-1,3-butadiene ir
4.2.3.27 dimethylallyl diphosphate light-dependent regulation of the thylakoid membrane-bound enzyme, overview Salix discolor isoprene + diphosphate isoprene is a volatile product emmitted to the atmosphere ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.27 10
-
-
 Salix discolor