EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.99.5 | degradation | new tryptophan catabolic pathway in Burholderia cepacia J2315, formation of the intermediate 4-oxalocrotonate differentiates this pathway from the proposed mammalian pathway which converts 2-aminomuconate to 2-ketoadipate and, ultimately, glutaryl-coenzyme A | Burkholderia cepacia |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.32 | expression as His-tag fusion protein in Escherichia coli Tuner (DE3) | Burkholderia cepacia |
1.2.1.32 | into the plasmid pDESTF1, which encodes an N-terminal six-His tag and is under control of the T7lac promoter, is used to transform Escherichia coli Tuner DE3 | Burkholderia cepacia |
3.5.99.5 | cloned into plasmid pDESTF1 and overexpressed in Escherichia coli Tuner (DE3) | Burkholderia cepacia |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.32 | 57000 | - |
SDS-PAGE | Burkholderia cepacia |
1.2.1.32 | 57000 | - |
SDS-PAGE, recombinant protein | Burkholderia cepacia |
3.5.99.5 | 18000 | - |
overexpressed soluble protein | Burkholderia cepacia |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.32 | 2-aminomuconate 6-semialdehyde + NAD+ + H2O | Burkholderia cepacia | - |
2-aminomuconate + NADH + H+ | - |
? | |
1.2.1.32 | 2-aminomuconate 6-semialdehyde + NAD+ + H2O | Burkholderia cepacia J2315 | - |
2-aminomuconate + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.32 | Burkholderia cepacia | - |
- |
- |
1.2.1.32 | Burkholderia cepacia J2315 | - |
- |
- |
3.5.99.5 | Burkholderia cepacia | - |
- |
- |
3.5.99.5 | Burkholderia cepacia J2315 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.32 | by nickelnitrilotriacetic acid affinity chromatography | Burkholderia cepacia |
1.2.1.32 | Ni-affinity chromatography | Burkholderia cepacia |
3.5.99.5 | by nickel-nitrilotriacetic acid affinity chromatography | Burkholderia cepacia |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.32 | 2-aminomuconate 6-semialdehyde + NAD+ + H2O | - |
Burkholderia cepacia | 2-aminomuconate + NADH + H+ | - |
? | |
1.2.1.32 | 2-aminomuconate 6-semialdehyde + NAD+ + H2O | - |
Burkholderia cepacia J2315 | 2-aminomuconate + NADH + H+ | - |
? | |
1.2.1.32 | 2-aminomuconate 6-semialdehyde + NAD+ + H2O | - |
Burkholderia cepacia | 2-aminomuconate + NADH + 2 H+ | - |
? | |
1.2.1.32 | 2-aminomuconate 6-semialdehyde + NAD+ + H2O | - |
Burkholderia cepacia J2315 | 2-aminomuconate + NADH + 2 H+ | - |
? | |
3.5.99.5 | 2-aminomuconate + H2O | - |
Burkholderia cepacia | 4-oxalocrotonate + NH3 | - |
? | |
3.5.99.5 | 2-aminomuconate + H2O | - |
Burkholderia cepacia J2315 | 4-oxalocrotonate + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.32 | 2-aminomuconate semialdehyde dehydrogenase | - |
Burkholderia cepacia |
1.2.1.32 | AMDH | - |
Burkholderia cepacia |
1.2.1.32 | aminomuconate-semialdehyde dehydrogenase | - |
Burkholderia cepacia |
3.5.99.5 | 2-aminomuconate deaminase | - |
Burkholderia cepacia |
3.5.99.5 | AMD | - |
Burkholderia cepacia |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.32 | NAD+ | - |
Burkholderia cepacia |