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Literature summary extracted from

  • Dabir, S.; Dabir, P.; Somvanshi, B.
    Purification, properties and alternate substrate specificities of arginase from two different sources: Vigna catjang cotyledon and buffalo liver (2005), Int. J. Biol. Sci., 1, 114-122.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.1 2
-
 L-arginine pH 9.2, 37°C Bos taurus
3.5.3.1 42
-
 L-arginine pH 10.0, 37°C Vigna catjang

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.1 Ca2+ 52% of the activity with Mn2+ Bos taurus
3.5.3.1 Ca2+ 35% of the activity with Mn2+ Vigna catjang
3.5.3.1 Co2+ 45% of the activity with Mn2+ Bos taurus
3.5.3.1 Co2+ 66% of the activity with Mn2+ Vigna catjang
3.5.3.1 Fe2+ 52% of the activity with Mn2+ Vigna catjang
3.5.3.1 Mg2+ 61% of the activity with Mn2+ Vigna catjang
3.5.3.1 Mg2+ 93% of the activity with Mn2+ Bos taurus
3.5.3.1 Mn2+ maximal activity at 0.6 mM Vigna catjang
3.5.3.1 Mn2+ maximal activity at 2.0 mM Bos taurus
3.5.3.1 additional information no activity with Fe2+, Zn2+ Bos taurus
3.5.3.1 Ni2+ 51% of the activity with Mn2+ Bos taurus
3.5.3.1 Ni2+ 55% of the activity with Mn2+ Vigna catjang
3.5.3.1 Zn2+ 38% of the activity with Mn2+ Vigna catjang

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.1 43000
-
 x * 43000, SDS-PAGE Bos taurus
3.5.3.1 52000
-
 4 * 52000, SDS-PAGE Vigna catjang
3.5.3.1 120000
-
 gel filtration Bos taurus
3.5.3.1 210000
-
 gel filtration Vigna catjang

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.1 Bos taurus
-
-
-
3.5.3.1 Vigna catjang
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.1
-
 Bos taurus
3.5.3.1
-
 Vigna catjang

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.1 cotyledon
-
 Vigna catjang
-
3.5.3.1 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.1 2045
-
 pH 10.0, 37°C Vigna catjang
3.5.3.1 91300
-
 pH 9.2, 37°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.1 agmatine + H2O 16% of the activity with L-arginine Vigna catjang 1,4-diaminobutane + urea
-
 ?
3.5.3.1 canavanine + H2O 12.7% of the activity with L-arginine Vigna catjang urea + NH2OCH2CH2CH(NH2)COOH
-
 ?
3.5.3.1 canavanine + H2O 9.6% of the activity with L-arginine Bos taurus urea + NH2OCH2CH2CH(NH2)COOH
-
 ?
3.5.3.1 L-arginine + H2O
-
 Bos taurus L-ornithine + urea
-
 ?
3.5.3.1 L-arginine + H2O
-
 Vigna catjang L-ornithine + urea
-
 ?
3.5.3.1 additional information no substrate: agmatine, D-arginine, L-homoarginine, L-argininic acid, gamma-guanidinobutyric acid, beta-guanidinopropionic acid, streptomycin Bos taurus ?
-
 ?
3.5.3.1 additional information no substrate: D-arginine, L-homoarginine, L-argininic acid, gamma-guanidinobutyric acid, beta-guanidinopropionic acid, streptomycin Vigna catjang ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.1 ? x * 43000, SDS-PAGE Bos taurus
3.5.3.1 tetramer 4 * 52000, SDS-PAGE Vigna catjang

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.1 35
-
-
 Vigna catjang
3.5.3.1 42
-
-
 Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.1 60
-
 stable Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.1 10
-
-
 Bos taurus
3.5.3.1 10
-
-
 Vigna catjang