EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.24 | gene cgh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain BL21(DE3) | Brucella abortus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.24 | additional information | construction of an isogenic deletion mutant strain DELTAcgh, the growth of the the cgh mutant is inhibited by addition of 5% or 10% bile acid, but is similar to the wild-type strain in absence of bile acids, the mutant enzyme does not confer resistance to bile acids to the organism | Brucella abortus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.24 | additional information | Brucella abortus | the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts | ? | - |
? | |
3.5.1.24 | additional information | Brucella abortus 2308 | the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.24 | Brucella abortus | Q2YS20 | - |
- |
3.5.1.24 | Brucella abortus 2308 | Q2YS20 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.24 | recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, and polymyxin B resin chromatography, the latter to eliminate lipopolysaccharide contamination | Brucella abortus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.24 | glycocholate + H2O | - |
Brucella abortus | glycine + cholate | - |
? | |
3.5.1.24 | glycocholate + H2O | - |
Brucella abortus 2308 | glycine + cholate | - |
? | |
3.5.1.24 | additional information | the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts | Brucella abortus | ? | - |
? | |
3.5.1.24 | additional information | the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts | Brucella abortus 2308 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.24 | bile salt hydrolase | - |
Brucella abortus |
3.5.1.24 | CGH | - |
Brucella abortus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.24 | 37 | - |
assay at | Brucella abortus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.24 | 7 | 8 | - |
Brucella abortus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.24 | 6 | 9 | activity range | Brucella abortus |